Corinna Kehrenberg1, Stefan Schwarz. 1. Institut für Tierzucht und Tierverhalten der Bundesforschungsanstalt für Landwirtschaft (FAL), Dörnbergstrasse 25-27, 29223 Celle, Germany.
Abstract
OBJECTIVES: A small resistance plasmid of Mannheimia varigena was analysed with regard to its gene organization and the development of a multiresistance gene cluster. MATERIALS AND METHODS: The 5621 bp plasmid pMVSCS1 was transformed into Escherichia coli JM107, cloned and sequenced completely. RESULTS: Three intact resistance genes, sulII, catAIII and strA, a truncated strB gene and a novel replication gene were identified. A potential recombination site for the integration of catAIII in the spacer region between sulII and strA was identified. CONCLUSION: The physical linkage of the plasmid-borne resistance genes organized in a cluster would facilitate the spread of the different resistance genes by co-selection, even in the absence of a direct selective pressure.
OBJECTIVES: A small resistance plasmid of Mannheimia varigena was analysed with regard to its gene organization and the development of a multiresistance gene cluster. MATERIALS AND METHODS: The 5621 bp plasmid pMVSCS1 was transformed into Escherichia coli JM107, cloned and sequenced completely. RESULTS: Three intact resistance genes, sulII, catAIII and strA, a truncated strB gene and a novel replication gene were identified. A potential recombination site for the integration of catAIII in the spacer region between sulII and strA was identified. CONCLUSION: The physical linkage of the plasmid-borne resistance genes organized in a cluster would facilitate the spread of the different resistance genes by co-selection, even in the absence of a direct selective pressure.
Authors: Janine T Bossé; Yanwen Li; Tom G Atherton; Stephanie Walker; Susanna M Williamson; Jon Rogers; Roy R Chaudhuri; Lucy A Weinert; Matthew T G Holden; Duncan J Maskell; Alexander W Tucker; Brendan W Wren; Andrew N Rycroft; Paul R Langford Journal: Vet Microbiol Date: 2015-05-28 Impact factor: 3.293
Authors: Alfonso Miranda; Bárbara Ávila; Patricia Díaz; Lina Rivas; Karen Bravo; Javier Astudillo; Constanza Bueno; María T Ulloa; Germán Hermosilla; Felipe Del Canto; Juan C Salazar; Cecilia S Toro Journal: Front Cell Infect Microbiol Date: 2016-07-20 Impact factor: 5.293