C Wang1, Z Li, K Wu. 1. Department of Biochemistry, Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
Abstract
OBJECTIVE: To study the rules of Streptococcus mutans reactive oxygen species metabolism and its enzymatic basis, and to investigate its significance intermediate to plaque microbial ecology. METHODS: Electron spin trapping method was used to measure reactive oxygen species produced by Streptococcus mutans in the different incubation conditions, and evaluate the efficicay of NADH to hypoxanthine-xanthine oxidase and Fe(2+)-H2O2 system. RESULTS: Electron spin resonance (ESR) spectrum of DMPO-O2- and DMPO-OH. and superoxide dismutase were obtained in Streptococcus mutans culture fluid, these spectrum were strengthened by exogenous sugar, and disappeared when methylene blue was added to culture fluid, but that spectrum was not significantly changed by NADH. ESR spectrum of hypoxanthine-xanthine oxidase and Fe(2+)-H2O2 system were significantly reduced or disappeared by NADH. CONCLUSION: These results suggest that there are O2- and OH in Streptococcus mutans metabolism, and they may come mainly from the reaction catalyzed by NADH oxidase.
OBJECTIVE: To study the rules of Streptococcus mutansreactive oxygen species metabolism and its enzymatic basis, and to investigate its significance intermediate to plaque microbial ecology. METHODS: Electron spin trapping method was used to measure reactive oxygen species produced by Streptococcus mutans in the different incubation conditions, and evaluate the efficicay of NADH to hypoxanthine-xanthine oxidase and Fe(2+)-H2O2 system. RESULTS: Electron spin resonance (ESR) spectrum of DMPO-O2- and DMPO-OH. and superoxide dismutase were obtained in Streptococcus mutans culture fluid, these spectrum were strengthened by exogenous sugar, and disappeared when methylene blue was added to culture fluid, but that spectrum was not significantly changed by NADH. ESR spectrum of hypoxanthine-xanthine oxidase and Fe(2+)-H2O2 system were significantly reduced or disappeared by NADH. CONCLUSION: These results suggest that there are O2- and OH in Streptococcus mutans metabolism, and they may come mainly from the reaction catalyzed by NADH oxidase.
Authors: Mária Džunková; Daniel Martinez-Martinez; Roman Gardlík; Michal Behuliak; Katarína Janšáková; Nuria Jiménez; Jorge F Vázquez-Castellanos; Jose Manuel Martí; Giuseppe D'Auria; H M H N Bandara; Amparo Latorre; Peter Celec; Andrés Moya Journal: NPJ Biofilms Microbiomes Date: 2018-11-27 Impact factor: 7.290