OBJECTIVE: Umbilical cord blood is a source of hematopoietic stem cells for transplantation. Although the first clinical applications have been encouraging, concern has been raised about contamination of umbilical blood by maternal cells, which might constitute a theoretical risk of graft-versus-host disease. The aim of this study was to assess the frequency of maternal deoxyribonucleic acid (DNA) contamination in umbilical cord plasma by using fluorescent polymerase chain reaction amplification of highly polymorphic short tandem repeat DNA markers. STUDY DESIGN: Fifty-seven mother/child pairs were tested for the presence of maternal DNA sequences in cord plasma. After delivery, cord blood samples were collected via gravity. Maternal specific alleles were detected by using polymerase chain reaction amplification of 9 highly polymorphic short tandem repeat markers (D21S11, D21S1411, D21S1412, D18S386, D18S535, MBP-A, MBP-B, D13S631, and D13S634). RESULTS: All 57 mother-child pairs were informative for the identification of uniquely maternal alleles in at least 2 of 9 different short tandem repeat markers used per case. Uniquely maternal DNA sequences were found in 43 of 57 (75%) cord plasma samples. CONCLUSION: The results of our study demonstrate that maternal DNA is present in the majority of umbilical cord blood plasma samples. The technique described herein might have application in the screening of umbilical cord blood samples for the presence of contaminating maternal genetic material.
OBJECTIVE: Umbilical cord blood is a source of hematopoietic stem cells for transplantation. Although the first clinical applications have been encouraging, concern has been raised about contamination of umbilical blood by maternal cells, which might constitute a theoretical risk of graft-versus-host disease. The aim of this study was to assess the frequency of maternal deoxyribonucleic acid (DNA) contamination in umbilical cord plasma by using fluorescent polymerase chain reaction amplification of highly polymorphic short tandem repeat DNA markers. STUDY DESIGN: Fifty-seven mother/child pairs were tested for the presence of maternal DNA sequences in cord plasma. After delivery, cord blood samples were collected via gravity. Maternal specific alleles were detected by using polymerase chain reaction amplification of 9 highly polymorphic short tandem repeat markers (D21S11, D21S1411, D21S1412, D18S386, D18S535, MBP-A, MBP-B, D13S631, and D13S634). RESULTS: All 57 mother-child pairs were informative for the identification of uniquely maternal alleles in at least 2 of 9 different short tandem repeat markers used per case. Uniquely maternal DNA sequences were found in 43 of 57 (75%) cord plasma samples. CONCLUSION: The results of our study demonstrate that maternal DNA is present in the majority of umbilical cord blood plasma samples. The technique described herein might have application in the screening of umbilical cord blood samples for the presence of contaminating maternal genetic material.
Authors: Nisha M Fernandes; Glenn P Taylor; Cedric Manlhiot; Brian W McCrindle; Michael Ho; Steven E S Miner; Adelle Atkinson; Edgar T Jaeggi; Lynne E Nield Journal: Pediatr Cardiol Date: 2011-04-12 Impact factor: 1.655
Authors: Eilis Hannon; Diana Schendel; Christine Ladd-Acosta; Jakob Grove; Christine Søholm Hansen; David Michael Hougaard; Michaeline Bresnahan; Ole Mors; Mads Vilhelm Hollegaard; Marie Bækvad-Hansen; Mady Hornig; Preben Bo Mortensen; Anders D Børglum; Thomas Werge; Marianne Giørtz Pedersen; Merete Nordentoft; Joseph D Buxbaum; M Daniele Fallin; Jonas Bybjerg-Grauholm; Abraham Reichenberg; Jonathan Mill Journal: Philos Trans R Soc Lond B Biol Sci Date: 2019-04-15 Impact factor: 6.237
Authors: Laurent Meric de Bellefon; Pierre Heiman; Sami B Kanaan; Doua F Azzouz; Justyna M Rak; Marielle Martin; Jean Roudier; Florence Roufosse; Nathalie C Lambert Journal: Chimerism Date: 2010-10
Authors: Alexander M Morin; Evan Gatev; Lisa M McEwen; Julia L MacIsaac; David T S Lin; Nastassja Koen; Darina Czamara; Katri Räikkönen; Heather J Zar; Karestan Koenen; Dan J Stein; Michael S Kobor; Meaghan J Jones Journal: Clin Epigenetics Date: 2017-07-25 Impact factor: 6.551