Literature DB >> 1180896

Purification of porcine enterokinase by affinity chromatography.

D A Grant, J Hermon-Taylor.   

Abstract

A method is described for the purification of porcine enterokinase by affinity chromatography with p-aminobenzamidine as the ligand. Purification was completed by immunoadsorption with antisera raised to components binding non-biologically to the gel. The final enterokinase preparation was 2.3 times more active than the most active preparation previously described.

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Year:  1975        PMID： 1180896      PMCID： PMC1165451          DOI： 10.1042/bj1470363

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  A spectrophotometric determination of trypsin and chymotrypsin.

Authors:  G W SCHWERT; Y TAKENAKA
Journal:  Biochim Biophys Acta       Date:  1955-04

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Studies on intestinal disaccharidases. V. Characterization and properties of maltase fractions from monkey intestine.

Authors:  N Swaminathan; A N Radhakrishnan
Journal:  Indian J Biochem       Date:  1970-03

4.  Affinity chromatography: purification of bovine trypsin and thrombin.

Authors:  H F Hixson; A H Nishikawa
Journal:  Arch Biochem Biophys       Date:  1973-02       Impact factor: 4.013

5.  Effect of ionic strength and calcium ions on the activation of trypsinogen by enterokinase. A modified test for the quantitative evaluation of this enzyme.

Authors:  J Baratti; S Maroux; D Louvard
Journal:  Biochim Biophys Acta       Date:  1973-10-10

6.  Inhibitory effect of aromatic diamidines on trypsin and enterokinase.

Authors:  J D Geratz
Journal:  Experientia       Date:  1969-12-15

7.  Assay of intestinal disaccharidases.

Authors:  A Dahlqvist
Journal:  Anal Biochem       Date:  1968-01       Impact factor: 3.365

8.  Purification and specificity of porcine enterokinase.

Authors:  S Maroux; J Baratti; P Desnuelle
Journal:  J Biol Chem       Date:  1971-08-25       Impact factor: 5.157

9.  On porcine enterokinase. Further purification and some molecular properties.

Authors:  J Baratti; S Maroux; D Louvard; P Desnuelle
Journal:  Biochim Biophys Acta       Date:  1973-07-05

10.  Single step purification of ovine luteinizing hormone by affinity chromatography.

Authors:  D Gospodarowicz
Journal:  J Biol Chem       Date:  1972-10-25       Impact factor: 5.157
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  5 in total

1.  Monoclonal antibody against human enterokinase and immunohistochemical localization of the enzyme.

Authors:  Y Miyoshi; T Onishi; T Sano; N Komi
Journal:  Gastroenterol Jpn       Date:  1990-06

2.  The purification of human enterokinase by affinity chromatography and immunoadsorption. Some observations on its molecular characteristics and comparisons with the pig enzyme.

Authors:  D A Grant; J Hermon-Taylor
Journal:  Biochem J       Date:  1976-05-01       Impact factor: 3.857

3.  The apparent molecular weights of human intestinal aminopeptidase, enterokinase and maltase in native duodenal fluid.

Authors:  A I Magee; D A Grant; J Hermon-Taylor
Journal:  Biochem J       Date:  1977-09-01       Impact factor: 3.857

4.  Immunofluorescent localisation of enterokinase in human small intestine.

Authors:  J Hermon-Taylor; J Perrin; D A Grant; A Appleyard; M Bubel; A I Magee
Journal:  Gut       Date:  1977-04       Impact factor: 23.059

Review 5.  Acute necrotising pancreatitis--a role for enterokinase.

Authors:  D Grant
Journal:  Int J Pancreatol       Date:  1986-10
  5 in total

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