Igor Kopac1, Milan Sterle, Ljubo Marion. 1. Dental Department, Centre of Fixed Prosthodontics and Occlusion, Medical Faculty, University of Ljubljana, Slovenia.
Abstract
STATEMENT OF PROBLEM: Chemical retraction agents used in fixed prosthodontics for temporary displacement of free gingival tissue before impression making can cause injury to the gingival tissue cells. PURPOSE: This study evaluated changes in cultured rat keratinocytes treated with 2 chemical agents used for gingival retraction. Treated cultures were compared with untreated cultures. MATERIAL AND METHODS: Keratinocytes of rat gingiva were grown in a specific medium for 10 days. After treating 1 group of specimens with 0.05% tetrahydrozoline and another group with 25% aluminum chloride, both for 10 minutes, the cultured cells were examined with scanning and transmission electron microscopy and compared with control specimens. RESULTS: Twenty-five percent aluminum chloride produced a significantly greater extent of cellular damage than 0.05% tetrahydrozoline, which caused only mild changes in the cultured cells. CONCLUSION: On the basis of the morphologic and ultrastructural changes in primary cell cultures of rat keratinocytes observed in this study, it was concluded that 25% aluminum chloride was significantly more aggressive than 0.05% tetrahydrozoline.
STATEMENT OF PROBLEM: Chemical retraction agents used in fixed prosthodontics for temporary displacement of free gingival tissue before impression making can cause injury to the gingival tissue cells. PURPOSE: This study evaluated changes in cultured rat keratinocytes treated with 2 chemical agents used for gingival retraction. Treated cultures were compared with untreated cultures. MATERIAL AND METHODS: Keratinocytes of rat gingiva were grown in a specific medium for 10 days. After treating 1 group of specimens with 0.05% tetrahydrozoline and another group with 25% aluminum chloride, both for 10 minutes, the cultured cells were examined with scanning and transmission electron microscopy and compared with control specimens. RESULTS: Twenty-five percent aluminum chloride produced a significantly greater extent of cellular damage than 0.05% tetrahydrozoline, which caused only mild changes in the cultured cells. CONCLUSION: On the basis of the morphologic and ultrastructural changes in primary cell cultures of rat keratinocytes observed in this study, it was concluded that 25% aluminum chloride was significantly more aggressive than 0.05% tetrahydrozoline.
Authors: Marko Igic; Milena Kostic; Jelena Basic; Nebojsa Krunic; Ana Pejcic; Nikola Gligorijevic; Aleksandra Milic Lemic Journal: Med Princ Pract Date: 2020-04-02 Impact factor: 1.927