Literature DB >> 11807293

Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility.

H B Dincturk1, A J Dunn, D H Jones.   

Abstract

The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11807293     DOI： 10.1007/bf02704761

Source DB:  PubMed          Journal:  J Biosci        ISSN： 0250-5991            Impact factor:   1.826


  14 in total

1.  The covalent and three-dimensional structure of concanavalin A. II. Amino acid sequence of cyanogen bromide fragment F3.

Authors:  B A Cunningham; J L Wang; M J Waxdal; G M Edelman
Journal:  J Biol Chem       Date:  1975-02-25       Impact factor: 5.157

2.  cDNAs for canavalin and concanavalin A from Canavalia gladiata seeds. Nucleotide sequence of cDNA for canavalin and RNA blot analysis of canavalin and concanavalin A mRNAs in developing seeds.

Authors:  D Yamauchi; K Nakamura; T Asahi; T Minamikawa
Journal:  Eur J Biochem       Date:  1988-01-04

3.  "ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli.

Authors:  E Amann; J Brosius
Journal:  Gene       Date:  1985       Impact factor: 3.688

4.  Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells.

Authors:  D Yamauchi; T Minamikawa
Journal:  FEBS Lett       Date:  1990-01-15       Impact factor: 4.124

5.  In vitro splicing of concanavalin A is catalyzed by asparaginyl endopeptidase.

Authors:  W Min; D H Jones
Journal:  Nat Struct Biol       Date:  1994-08

6.  Heterogeneity of concanavalin A as detected by its binding to p-nitrophenyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside.

Authors:  T J Williams; J A Shafer; I J Goldstein
Journal:  J Biol Chem       Date:  1978-12-10       Impact factor: 5.157

7.  Expression of a wheat alpha-amylase gene in Escherichia coli: recognition of the translational initiation site and the signal peptide.

Authors:  A A Gatenby; M Boccara; D C Baulcombe; S J Rothstein
Journal:  Gene       Date:  1986       Impact factor: 3.688

8.  Production of pea lectin in Escherichia coli.

Authors:  M E Stubbs; J P Carver; R J Dunn
Journal:  J Biol Chem       Date:  1986-05-15       Impact factor: 5.157

9.  Non-glycosylated recombinant pro-concanavalin A is active without polypeptide cleavage.

Authors:  W Min; A J Dunn; D H Jones
Journal:  EMBO J       Date:  1992-04       Impact factor: 11.598

Review 10.  Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.

Authors:  M Kozak
Journal:  Microbiol Rev       Date:  1983-03
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.