| Literature DB >> 11807086 |
Li-Fong Lee1, Shun-Hua Yeh, Carton W Chen.
Abstract
Temperature-sensitive mutants of Streptomyces defective in initiation of chromosome replication were created by in vitro site-directed mutagenesis in the dnaA gene followed by gene replacement. When they were shifted to 39 degrees C replication in the mutants ceased in about 90 min but resumed on return to 30 degrees C. This allowed manipulations to achieve replication synchronization.Mesh:
Substances:
Year: 2002 PMID: 11807086 PMCID: PMC134811 DOI: 10.1128/jb.184.4.1214-1218.2002
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490