J Wang1, C Song, S Wang. 1. Biochemical Lab, Beijing Tuberculosis & Thoracic Tumor Research Institute, Beijing 101149, China.
Abstract
OBJECTIVE: To assess the association of HLA-DRB1 genes with pulmonary tuberculosis (PTB) and the correlation between HLA-DRB1 alleles and clinical manifestations of patients with PTB. METHOD: Alleles at the HLA-DRB1 typing were analyzed by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 74 patients and 90 healthy controls. RESULTS: Compared with controls, a significant increased frequency of DRB1 * 15 was found (34.3% vs 17.0%, Pc < 0.05, RR = 2.91). The frequency of DRB1 * 12 was also increased, but the difference was not statistically significant(15.4% vs 7.5%, Pc > 0.05). The frequency of DRB1 * 11 was significantly decreased (1.4% vs 9.9%, Pc < 0.05, RR = 0.12). More retreated and drug-resistant cases were found in DRB1 * 15 positive patients than in DRB1 * 15 negative patients. CONCLUSIONS: These results show that HLA-DRB1 * 15 allele is susceptible gene in PTB, DRB1 * 11 may be a protective allele, HLA-DRB1 * 15 allele may be related to patients with refractory PTB.
OBJECTIVE: To assess the association of HLA-DRB1 genes with pulmonary tuberculosis (PTB) and the correlation between HLA-DRB1 alleles and clinical manifestations of patients with PTB. METHOD: Alleles at the HLA-DRB1 typing were analyzed by polymerase chain reaction with sequence-specific primers (PCR-SSP) in 74 patients and 90 healthy controls. RESULTS: Compared with controls, a significant increased frequency of DRB1 * 15 was found (34.3% vs 17.0%, Pc < 0.05, RR = 2.91). The frequency of DRB1 * 12 was also increased, but the difference was not statistically significant(15.4% vs 7.5%, Pc > 0.05). The frequency of DRB1 * 11 was significantly decreased (1.4% vs 9.9%, Pc < 0.05, RR = 0.12). More retreated and drug-resistant cases were found in DRB1 * 15 positive patients than in DRB1 * 15 negative patients. CONCLUSIONS: These results show that HLA-DRB1 * 15 allele is susceptible gene in PTB, DRB1 * 11 may be a protective allele, HLA-DRB1 * 15 allele may be related to patients with refractory PTB.