X Ni1, Y Chen. 1. Institute of Infectious and Parasitic Diseases, College of Clinical Medicine, Chongqing University of Medicine Sciences, Chongqing 400016, China.
Abstract
OBJECTIVE: In vitro study was carried out to assess the effect of dihydroarteminsinin (DHA) and sodium artesunate (ATS) on pneumocystis carinii (Pc). METHODS: The drugs were added into HepG-2 cell cultures containing Pc cysts. The concentrations of dihydroarteminsinin (DHA) were 100, 50, 10, 5, 0.5 micromol/L respectively. The concentrations of sodium artesunate (ATS) were 100, 50, 10, 5 micromol/L respectively and pentamidine (PTD) was 15 micromol/L as a control. The supernatants were withdrawn every other day and evaluated for Pc trophozoite and cyst counts by GMS and DQ stain. RESULTS: The peak of Pc multiplication was observed on 5th day. The inhibition rates of Pc trophozoites by DHA 50 micromol/L, 100 micromol/L and ATS 100 micromol/L were quite similar to that of pentamidine (PTD) 15 micromol/L. CONCLUSION: DHA and ATS can inhibit the growth of Pc as same as PTD, and DHA was slightly better than ATS.
OBJECTIVE: In vitro study was carried out to assess the effect of dihydroarteminsinin (DHA) and sodium artesunate (ATS) on pneumocystis carinii (Pc). METHODS: The drugs were added into HepG-2 cell cultures containing Pc cysts. The concentrations of dihydroarteminsinin (DHA) were 100, 50, 10, 5, 0.5 micromol/L respectively. The concentrations of sodium artesunate (ATS) were 100, 50, 10, 5 micromol/L respectively and pentamidine (PTD) was 15 micromol/L as a control. The supernatants were withdrawn every other day and evaluated for Pc trophozoite and cyst counts by GMS and DQ stain. RESULTS: The peak of Pc multiplication was observed on 5th day. The inhibition rates of Pc trophozoites by DHA 50 micromol/L, 100 micromol/L and ATS 100 micromol/L were quite similar to that of pentamidine (PTD) 15 micromol/L. CONCLUSION:DHA and ATS can inhibit the growth of Pc as same as PTD, and DHA was slightly better than ATS.