Production and characterization of extracellular and intracellular tannase from newly isolated Aspergillus aculeatus DBF 9.

A comparative study on the simultaneous production of extra and intracellular tannase was made from newly isolated fungal strain Aspergillus aculeatus DBF 9. This strain produced five times more intracellular enzyme within 24 h in liquid culture than the extracellular form. Maximum tannase production occurred in the culture broth containing 1-2% (w/v) tannic acid and 0.05-0.1% (w/v) glucose. The pH and temperature optima of both the enzymes were found at 5.0 and 50-60 degrees C, respectively. Extra and intracellular tannase showed good stability at higher temperature, pH values and salt (NaCl) concentration. These properties make the enzyme suitable for pollution control and bioprocess industry.