OBJECTIVES: A panel of cytokines has been found to be important for eosinophil accumulation and activation in nasal polyps. The aims of this study were to ascertain whether the activation of nuclear factor-kappa B (NF-kappaB) occurred in the polyp epithelium, and to examine the relationship between the degree of activation and local cytokine gene expression. STUDY DESIGN/ METHODS: Nasal polyp specimens were obtained from 26 untreated patients. The proportion of nuclear translocation of the NF-kappaB p50 subunit in the polyp epithelium was quantitatively analyzed by a combination of fluorescent immunohistochemistry and a laser scanning confocal microscope image system. The levels of GM-CSF, IL-5, IL-8, IL-16, and eotaxin mRNA expression in the same speci-mens were measured using reverse transcription-polymerase chain reaction. RESULTS: Both cytoplasmic and nuclear localization of the p50 subunit was observed mainly in the epithelial layer in all specimens. The percentages of epithelial cells with nuclear translocation ranged from 4.5% to 40.5% (median, 18%). Significant correlations were observed between the degree of epithelial NF-kappaB activation and the levels of IL-8, IL-16, and eotaxin mRNA expression (r = 0.468, 0.47, and 0.739, respectively). CONCLUSION: The activation of NF-kappaB in the nasal polyp epithelium is responsible for the recruitment of inflammatory cells, particularly eosinophils, through the initiation of the transcriptional pathway of the related cytokines. The increased NF-kappaB activity in the polyp epithelium may reflect hypersensitivity to unknown stimuli.
OBJECTIVES: A panel of cytokines has been found to be important for eosinophil accumulation and activation in nasal polyps. The aims of this study were to ascertain whether the activation of nuclear factor-kappa B (NF-kappaB) occurred in the polyp epithelium, and to examine the relationship between the degree of activation and local cytokine gene expression. STUDY DESIGN/ METHODS: Nasal polyp specimens were obtained from 26 untreated patients. The proportion of nuclear translocation of the NF-kappaB p50 subunit in the polyp epithelium was quantitatively analyzed by a combination of fluorescent immunohistochemistry and a laser scanning confocal microscope image system. The levels of GM-CSF, IL-5, IL-8, IL-16, and eotaxin mRNA expression in the same speci-mens were measured using reverse transcription-polymerase chain reaction. RESULTS: Both cytoplasmic and nuclear localization of the p50 subunit was observed mainly in the epithelial layer in all specimens. The percentages of epithelial cells with nuclear translocation ranged from 4.5% to 40.5% (median, 18%). Significant correlations were observed between the degree of epithelial NF-kappaB activation and the levels of IL-8, IL-16, and eotaxin mRNA expression (r = 0.468, 0.47, and 0.739, respectively). CONCLUSION: The activation of NF-kappaB in the nasal polyp epithelium is responsible for the recruitment of inflammatory cells, particularly eosinophils, through the initiation of the transcriptional pathway of the related cytokines. The increased NF-kappaB activity in the polyp epithelium may reflect hypersensitivity to unknown stimuli.
Authors: Andreas Lackner; Reinhard Bernd Raggam; Heinz Stammberger; Alfred Beham; Hannes Braun; Barbara Kleinhappl; Walter Buzina; Clemens Kittinger; Sabine Reinisch; Andrea Berghold; Kurt Freudenschuss; Sonja Barth; Egon Marth Journal: Eur Arch Otorhinolaryngol Date: 2007-04-13 Impact factor: 2.503
Authors: Hahn Jin Jung; Yu Lian Zhang; Dong Kyu Kim; Chae Seo Rhee; Dong Young Kim Journal: Allergy Asthma Immunol Res Date: 2019-11 Impact factor: 5.764
Authors: Ruowu Liu; Jintao Du; Jiao Zhou; Bing Zhong; Luo Ba; Jie Zhang; Yafeng Liu; Shixi Liu Journal: Front Immunol Date: 2021-04-15 Impact factor: 7.561
Authors: Fabiana Valera; María S Brassesco; Angel M Castro-Gamero; Maria A Cortez; Rosane G P Queiroz; Luiz G Tone; Wilma T Anselmo-Lima Journal: Braz J Otorhinolaryngol Date: 2011 Sep-Oct