F Lu1, X Li. 1. Department of Plastic Surgery, Nanfang Hospital of the First Military Medical University. Guangzhou 510515, China.
Abstract
OBJECTIVE: To explore the concrete mechanism which accounts for the different growth characteristics of normal skins and pathlogical scars and to identify if the mutations of Fas gene exist. METHODS: Six samples of normal skin, hypertrophic scars and keloids were collected. The means of cell culture was used, and only 6 - 10 passages fibroblasts were selected for experiment. The distribution of cell cycle and expression of protein p53 were analysed with flow cytometry, adherant cell analysis and sorting interactive laser coytometry (ACAS 570). Keloids and hypertrophic scar tissue and the peripheral blood of each patient were collected. After DNA was isolated from the samples, we used the single-stranded conformation polymorphism (SSCP) analysis and DNA sequencing to examine the structure of Fas gene. RESULTS: The expression of p53 protein on the fibroblasts derived from normal skin was significantly elevated in comparison to the hypertrophic scars with lowest propagative fibroblasts. A large percent of fibroblasts derived from the peripherial areas of keloids distributed in G(2), S and M periods with low levels of p53 protein. On the other hand, fibroblasts derived from the central areas of keloids distributed mostly in G(0) and G(1) periods with higher expression of p53 protein. Mutation in Fas gene was identified and confirmed in keloid tissue of 20% (2/10) of keloid patient, which may cause loss of function and contribute to the pathogenesis of keloid. A deletion of the nucleotide "A" at the same position was found, which caused a frameshift mutation in exon 9. CONCLUSION: The difference of the distributions of cell cycle and expression of protein p53 may account for the different growth characteristics in pathlogical scar and normal skin. Mutations in Fas gene may lead to abnormal apoptosis of fibroblasts in keloids, and may contribute to the pathogenesis and progression of keloids.
OBJECTIVE: To explore the concrete mechanism which accounts for the different growth characteristics of normal skins and pathlogical scars and to identify if the mutations of Fas gene exist. METHODS: Six samples of normal skin, hypertrophic scars and keloids were collected. The means of cell culture was used, and only 6 - 10 passages fibroblasts were selected for experiment. The distribution of cell cycle and expression of protein p53 were analysed with flow cytometry, adherant cell analysis and sorting interactive laser coytometry (ACAS 570). Keloids and hypertrophic scar tissue and the peripheral blood of each patient were collected. After DNA was isolated from the samples, we used the single-stranded conformation polymorphism (SSCP) analysis and DNA sequencing to examine the structure of Fas gene. RESULTS: The expression of p53 protein on the fibroblasts derived from normal skin was significantly elevated in comparison to the hypertrophic scars with lowest propagative fibroblasts. A large percent of fibroblasts derived from the peripherial areas of keloids distributed in G(2), S and M periods with low levels of p53 protein. On the other hand, fibroblasts derived from the central areas of keloids distributed mostly in G(0) and G(1) periods with higher expression of p53 protein. Mutation in Fas gene was identified and confirmed in keloid tissue of 20% (2/10) of keloid patient, which may cause loss of function and contribute to the pathogenesis of keloid. A deletion of the nucleotide "A" at the same position was found, which caused a frameshift mutation in exon 9. CONCLUSION: The difference of the distributions of cell cycle and expression of protein p53 may account for the different growth characteristics in pathlogical scar and normal skin. Mutations in Fas gene may lead to abnormal apoptosis of fibroblasts in keloids, and may contribute to the pathogenesis and progression of keloids.