J Wang1, L Cao, M He. 1. The First Hospital, Beijing Medical University, Beijing 100034, China.
Abstract
OBJECTIVE: To explore the flow cytometric 3-color analysis of circulating activated platelets (CAP) and its clinical significance in ischemic cerebrovascular diseases. METHODS: The fibrinogen receptor (FIB-R) and P-selectin (CD62P) were used for molecular marker of CAP, which were analyzed by flow cytometric 3-color immunofluorescence. RESULTS: Expression of FIB-R and CD62P on the resting platelet membrane surface was very lower in normal individuals, the median of FIB-R and CD62P positive percent was 1.40% and 0.70% respectively. Platelet can be obviously activated by 0.1 micromol/L ADP, and 10.0 micromol/L ADP leaded to the peak expression of FIB-R and CD62P on the activated platelet membrane surface, FIB-R and CD62P positive percent of 87.8% and 81.34% respectively. That monoclonal antibody of the FIB-R combined with ADP activating platelets was inhibited by synthetic peptide (RGDS). The quantity of CAP with FIB-R expression was markedly higher (P < 0.01) and the amount of CAP with CD62P expression was not significantly higher (P > 0.05) in blood of 112 patients with thrombotic tendency and 120 patients with ischemic cerebrovascular diseases than in healthy individuals. The expression of CAP with FIB-R of in front and behind treatment of acute cerebral infarction continuously decreased (P < 0.05), and the quantity of CAP with FIB-R expression was related negatively to Europe stroke score (ESS) of patients with acute cerebral infarction. CONCLUSION: The FIB-R may be regarded as sensitive and specific molecular marker in detection of CAP. The level of platelet activation in vivo can be reflected accurately by flow cytometric 3-color analysis of CAP, which has an important clinical significance for the study of pathogenesis, estimation of prognosis and supervision of curative effect in ischemic cerebrovascular diseases.
OBJECTIVE: To explore the flow cytometric 3-color analysis of circulating activated platelets (CAP) and its clinical significance in ischemic cerebrovascular diseases. METHODS: The fibrinogen receptor (FIB-R) and P-selectin (CD62P) were used for molecular marker of CAP, which were analyzed by flow cytometric 3-color immunofluorescence. RESULTS: Expression of FIB-R and CD62P on the resting platelet membrane surface was very lower in normal individuals, the median of FIB-R and CD62P positive percent was 1.40% and 0.70% respectively. Platelet can be obviously activated by 0.1 micromol/L ADP, and 10.0 micromol/L ADP leaded to the peak expression of FIB-R and CD62P on the activated platelet membrane surface, FIB-R and CD62P positive percent of 87.8% and 81.34% respectively. That monoclonal antibody of the FIB-R combined with ADP activating platelets was inhibited by synthetic peptide (RGDS). The quantity of CAP with FIB-R expression was markedly higher (P < 0.01) and the amount of CAP with CD62P expression was not significantly higher (P > 0.05) in blood of 112 patients with thrombotic tendency and 120 patients with ischemic cerebrovascular diseases than in healthy individuals. The expression of CAP with FIB-R of in front and behind treatment of acute cerebral infarction continuously decreased (P < 0.05), and the quantity of CAP with FIB-R expression was related negatively to Europe stroke score (ESS) of patients with acute cerebral infarction. CONCLUSION: The FIB-R may be regarded as sensitive and specific molecular marker in detection of CAP. The level of platelet activation in vivo can be reflected accurately by flow cytometric 3-color analysis of CAP, which has an important clinical significance for the study of pathogenesis, estimation of prognosis and supervision of curative effect in ischemic cerebrovascular diseases.
Authors: Hai Bin He; Xian Lin Wu; Bin Yu; Kang Li Liu; Guang Xiong Zhou; Guo Qiang Qian; Da Hong Ju; Xiao Yin Chen Journal: Hepat Mon Date: 2011-05 Impact factor: 0.660