X Liu1, T Yu, M Zhao, X Tang, Q Gu, N Liu. 1. Department of Internal Medicine, First Teaching Hospital of Beijing Medical University, Beijing 100034.
Abstract
OBJECTIVE: To inquire into the diagnostic significance of antineutrophil cytoplasmic antibodies (ANCA) in ulcerative colitis (UC). METHODS: Serum ANCA from 58 UC patients, 43 non-UC patients and 58 healthy donors were detected with indirect immunofluorescence (IIF), enzyme linked immunosorbent assay (ELISA)and Western blot. RESULTS: The sensitivity and specificity of the presence of ANCA in the diagnosis of UC were 37.93% and 100% respectively. In the group of UC, patients with mild, moderate and severe clinical manifestations had positive ANCA rates of 17.65%, 41.67% and 52.94% respectively. The occurrence rates of mucosal vasculitis and grade III-V mucosal inflammation were 78.95% and 78.95% respectively in ANCA-positive group, but 37.04% and 44.44% in ANCA-negative group. The levels of the binding of the five kinds of ANCA antigens i.e. myeloperoxidase (MPO), bactericidal/permeability increasing protein (BPI), lactoferrin (LF), cathepsin G (CG) and proteinase-3 (PR-3) with the serum of UC patients were 13.99%, 13.79%, 10.34% 10.34% and 8.62% respectively. Specific protein strips were observed in 48.28% of the UC patients with Western blot technique, 47,000 polypeptide being the most common and making up 22.41% of them. CONCLUSION: The detection of ANCA was an auxiliary method for the diagnosis of UC. At present, UC-associated target antigens were not yet clarified, 47,000 polypeptide may be one of them. ANCA may participate in the pathogenesis of UC.
OBJECTIVE: To inquire into the diagnostic significance of antineutrophil cytoplasmic antibodies (ANCA) in ulcerative colitis (UC). METHODS: Serum ANCA from 58 UC patients, 43 non-UC patients and 58 healthy donors were detected with indirect immunofluorescence (IIF), enzyme linked immunosorbent assay (ELISA)and Western blot. RESULTS: The sensitivity and specificity of the presence of ANCA in the diagnosis of UC were 37.93% and 100% respectively. In the group of UC, patients with mild, moderate and severe clinical manifestations had positive ANCA rates of 17.65%, 41.67% and 52.94% respectively. The occurrence rates of mucosal vasculitis and grade III-V mucosal inflammation were 78.95% and 78.95% respectively in ANCA-positive group, but 37.04% and 44.44% in ANCA-negative group. The levels of the binding of the five kinds of ANCA antigens i.e. myeloperoxidase (MPO), bactericidal/permeability increasing protein (BPI), lactoferrin (LF), cathepsin G (CG) and proteinase-3 (PR-3) with the serum of UC patients were 13.99%, 13.79%, 10.34% 10.34% and 8.62% respectively. Specific protein strips were observed in 48.28% of the UC patients with Western blot technique, 47,000 polypeptide being the most common and making up 22.41% of them. CONCLUSION: The detection of ANCA was an auxiliary method for the diagnosis of UC. At present, UC-associated target antigens were not yet clarified, 47,000 polypeptide may be one of them. ANCA may participate in the pathogenesis of UC.
Authors: Jung Yoon Pyo; Jin Su Park; Chang Ho Song; Sang Won Lee; Yong Beom Park; Soo Kon Lee Journal: Korean J Intern Med Date: 2013-07-01 Impact factor: 2.884