Literature DB >> 11796643

Conformationally correct expression of membrane-anchored Toxoplasma gondii SAG1 in the primitive protozoan Giardia duodenalis.

Matthias Marti1, Yajie Li, Peter Köhler, Adrian B Hehl.   

Abstract

To explore the possibility of expressing membrane-anchored exodomains of heterologous surface antigens in Giardia, a chimeric construct containing the Toxoplasma gondii SAG1 gene was made. The Giardia system is shown here to provide a means of generating correctly folded chimeric surface proteins in a native and unmodified form.

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Year:  2002        PMID： 11796643      PMCID： PMC127713          DOI： 10.1128/IAI.70.2.1014-1016.2002

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  16 in total

1.  Giardia lamblia: post-translational processing and status of exposed cysteine residues in TSA 417, a variable surface antigen.

Authors:  S B Aley; F D Gillin
Journal:  Exp Parasitol       Date:  1993-11       Impact factor: 2.011

2.  Purification of a major membrane protein of Toxoplasma gondii by immunoabsorption with a monoclonal antibody.

Authors:  L H Kasper; J H Crabb; E R Pfefferkorn
Journal:  J Immunol       Date:  1983-05       Impact factor: 5.422

3.  Stage-specific expression and targeting of cyst wall protein-green fluorescent protein chimeras in Giardia.

Authors:  A B Hehl; M Marti; P Köhler
Journal:  Mol Biol Cell       Date:  2000-05       Impact factor: 4.138

4.  Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay.

Authors:  D Aubert; G T Maine; I Villena; J C Hunt; L Howard; M Sheu; S Brojanac; L E Chovan; S F Nowlan; J M Pinon
Journal:  J Clin Microbiol       Date:  2000-03       Impact factor: 5.948

5.  Characterization of Toxoplasma gondii surface antigen 1 (SAG1) secreted from Pichia pastoris: evidence of hyper O-glycosylation.

Authors:  O Letourneur; G Gervasi; S Gaïa; J Pagès; B Watelet; M Jolivet
Journal:  Biotechnol Appl Biochem       Date:  2001-02       Impact factor: 2.431

6.  Isolation and expression of the gene for a major surface protein of Giardia lamblia.

Authors:  F D Gillin; P Hagblom; J Harwood; S B Aley; D S Reiner; M McCaffery; M So; D G Guiney
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 11.205

7.  Antigenic variation of Giardia lamblia in experimental human infections.

Authors:  T E Nash; D A Herrington; M M Levine; J T Conrad; J W Merritt
Journal:  J Immunol       Date:  1990-06-01       Impact factor: 5.422

8.  Variant surface antigens of Giardia lamblia are associated with the presence of a thick cell coat: thin section and label fracture immunocytochemistry survey.

Authors:  P F Pimenta; P P da Silva; T Nash
Journal:  Infect Immun       Date:  1991-11       Impact factor: 3.441

9.  Protection of mice from fatal Toxoplasma gondii infection by immunization with p30 antigen in liposomes.

Authors:  R Bülow; J C Boothroyd
Journal:  J Immunol       Date:  1991-11-15       Impact factor: 5.422

10.  Chronic giardiasis: studies on drug sensitivity, toxin production, and host immune response.

Authors:  P D Smith; F D Gillin; W M Spira; T E Nash
Journal:  Gastroenterology       Date:  1982-10       Impact factor: 22.682
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  2 in total

1.  The secretory apparatus of an ancient eukaryote: protein sorting to separate export pathways occurs before formation of transient Golgi-like compartments.

Authors:  Matthias Marti; Yajie Li; Elisabeth M Schraner; Peter Wild; Peter Köhler; Adrian B Hehl
Journal:  Mol Biol Cell       Date:  2003-04       Impact factor: 4.138

2.  Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity.

Authors:  Marina Clemente; Roberto Curilovic; Alina Sassone; Alicia Zelada; Sergio O Angel; Alejandro N Mentaberry
Journal:  Mol Biotechnol       Date:  2005-05       Impact factor: 2.695
  2 in total

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