| Literature DB >> 11795481 |
P O Vardevanyan1, A P Antonyan, G A Manukyan, A T Karapetyan.
Abstract
The helix-coil transition of DNA-ethidium bromide complexes in an interval of ionic strength of 2.0 x 10(-3) M < or = muNa+ < or = 2.0 x 10(-2) M has been investigated. It has been revealed that at the certain high ligand-DNA ratios (r(b)) the transition interval of the complex--(deltaT) becomes equal to that of DNA itself (deltaTo). It has been shown that the values of r(b) at which delta deltaT=deltaT-deltaT0=0 depends on ionic strength of a solution. Further increasing of ligand concentration leads to its conversion from stabilizer into the destabilizer of the double-stranded DNA.Entities:
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Year: 2001 PMID： 11795481 DOI： 10.1038/emm.2001.34
Source DB: PubMed Journal: Exp Mol Med ISSN： 1226-3613 Impact factor: 8.718