J Hu1, Q Wang, W Liang. 1. Guangzhou University of Traditional Chinese Medicine, Guangzhou (510405).
Abstract
OBJECTIVE: To observe the effect of Bushen Yizhi Recipe (BYD) on somatostatin-like (SS-like) immuno-reactivity and SS mRNA expressed-positive neurons in Alzheimer disease (AD) model rats induced by D-galactose intraperitoneal and ibotenic acid (IBO) intracerebral injection. METHODS: Fifteen months Wistar rats were randomly divided into 4 groups: normal control; AD model control induced by D-galactose intraperitoneal injection and lesion of basal nucleus of Meynert with IBO; Huperzine A (Hup-A) treated group and BYD treated group fed with Hup-A or BYD respectively. The SS-like immunoreactivity and SS mRNA expressed-positive neurons in parietal lobe, hippocampal CA1 field and hilus of the dentate gyrus in the brains of rats, which were detected with immunohistochemistry and DIG-labelling cRNA-probe in situ hybridization. RESULTS: Counts and integrations of optic density of SS-like immuno-reactivity and SS-mRNA expressed neurons in parietal lobe, hippocampal CA1 field and hilus of the dentate gyrus in BYD treated group increased compared with AD model rats. CONCLUSION: BYD might protect the SS-ergic system against impairment of D-galactose and IBO in the brains of the AD model rats.
OBJECTIVE: To observe the effect of Bushen Yizhi Recipe (BYD) on somatostatin-like (SS-like) immuno-reactivity and SS mRNA expressed-positive neurons in Alzheimer disease (AD) model rats induced by D-galactose intraperitoneal and ibotenic acid (IBO) intracerebral injection. METHODS: Fifteen months Wistar rats were randomly divided into 4 groups: normal control; AD model control induced by D-galactose intraperitoneal injection and lesion of basal nucleus of Meynert with IBO; Huperzine A (Hup-A) treated group and BYD treated group fed with Hup-A or BYD respectively. The SS-like immunoreactivity and SS mRNA expressed-positive neurons in parietal lobe, hippocampal CA1 field and hilus of the dentate gyrus in the brains of rats, which were detected with immunohistochemistry and DIG-labelling cRNA-probe in situ hybridization. RESULTS: Counts and integrations of optic density of SS-like immuno-reactivity and SS-mRNA expressed neurons in parietal lobe, hippocampal CA1 field and hilus of the dentate gyrus in BYD treated group increased compared with AD model rats. CONCLUSION: BYD might protect the SS-ergic system against impairment of D-galactose and IBO in the brains of the AD model rats.