OBJECTIVES: To determine the prognostic importance of AgNORs and ki-67 in premalignant and malignant lesions in the oral cavity, we carried out a study using retroprospective phases in which 98 histologic sections. STUDY DESIGN: 26 of mild epithelial dysplasia DEL, 15 of moderate epithelial dysplasia DEM, and 57 of invasive oral squamous cell carcinoma CECO, were stained with silver colloid stain to obtain the average number and type of AgNORs, and immunologic marker Ki-67 to obtain the percentage of cells Ki-67 (+). RESULTS: In both phases the average number of AgNORs and Ki-67 (+) cells was significantly greater in the CECO cases, compared to the dysplasias. The AgNORs type 1 were predominant in the DEL cases and the type 2 in the CECO cases. No statistically significant correlation (Sperman's simple range correlation) was found between the average numbers of AgNORS and the percentage of Ki-67 (+) cells in the three lesions studied. (DEL: p= 0.5113, DEM: p= 0.92 y CECO: p= 0.2584). CONCLUSIONS: The results suggest that the AgNOR tissue marker can be used as a routine complementary histopathologic study, since the variations in its number and distribtion indicate existence of cell alterations in a given lesion and the use of this technique is easy and inexpensive.
OBJECTIVES: To determine the prognostic importance of AgNORs and ki-67 in premalignant and malignant lesions in the oral cavity, we carried out a study using retroprospective phases in which 98 histologic sections. STUDY DESIGN: 26 of mild epithelial dysplasia DEL, 15 of moderate epithelial dysplasia DEM, and 57 of invasive oral squamous cell carcinoma CECO, were stained with silver colloid stain to obtain the average number and type of AgNORs, and immunologic marker Ki-67 to obtain the percentage of cells Ki-67 (+). RESULTS: In both phases the average number of AgNORs and Ki-67 (+) cells was significantly greater in the CECO cases, compared to the dysplasias. The AgNORs type 1 were predominant in the DEL cases and the type 2 in the CECO cases. No statistically significant correlation (Sperman's simple range correlation) was found between the average numbers of AgNORS and the percentage of Ki-67 (+) cells in the three lesions studied. (DEL: p= 0.5113, DEM: p= 0.92 y CECO: p= 0.2584). CONCLUSIONS: The results suggest that the AgNOR tissue marker can be used as a routine complementary histopathologic study, since the variations in its number and distribtion indicate existence of cell alterations in a given lesion and the use of this technique is easy and inexpensive.