Estrogen attenuates postexercise HSP70 expression in skeletal muscle.

Exercise has been demonstrated as a physiological inducer of heat shock protein (HSP)70. Many of the proposed signals of this response exhibit sexual dimorphism. Thus the present objectives were to determine whether HSP70 induction after exercise exhibits gender specificity and to elucidate the mechanisms underlying such a phenomenon. Postexercise HSP70 induction in skeletal muscle was greater in male than female rats at the level of protein and mRNA (P = 0.005). Moreover, placebo-treated ovariectomized animals demonstrated a greater HSP70 response to exercise than those treated with estrogen (P = 0.015 and 0.019 for protein and mRNA, respectively). These findings indicate that the gender-specific HSP70 response to exercise is mediated by the female-specific hormone estrogen. Compounds structurally related to 17beta-estradiol, the major endogenous estrogen, but which do not activate the estrogen receptor, also attenuated HSP70 induction with exercise (P < 0.01), indicating a nongenomic hormonal mechanism. These findings highlight a specific example of the biological differences between males and females and reiterate the physiological effects of sex hormones extending beyond their roles in reproductive function.