Q Tang1, R Yang, Y Han. 1. Department of Neurology, Affiliated Hospital of Bengbu Medical College, Anhui (233004).
Abstract
OBJECTIVE: To explore the cytological mechanism of Gandou Decoction (GDD) in treating hepatolenticular degeneration (HLD). METHODS: Twenty-three in vitro models of cultured skin fibroblast of HLD were established to observe the intracellular change of microelements (Cu2+, Zn2+, etc.) content before and after adding rabbit's serum containing GDD. RESULTS: After being treated with GDD contained serum for 24 hours, the intracellular content of Cu2+ decreased from (80.94 +/- 34.76) ng/mg to (46.90 +/- 22.14) ng/mg, P < 0.01, while that of Zn2+ increased from (140.43 +/- 33.81) ng/mg to (151.43 +/- 37.83) ng/mg, P < 0.01. CONCLUSION: GDD is quite effective in removing the intracellular copper and increasing the intracellular zinc.
OBJECTIVE: To explore the cytological mechanism of Gandou Decoction (GDD) in treating hepatolenticular degeneration (HLD). METHODS: Twenty-three in vitro models of cultured skin fibroblast of HLD were established to observe the intracellular change of microelements (Cu2+, Zn2+, etc.) content before and after adding rabbit's serum containing GDD. RESULTS: After being treated with GDD contained serum for 24 hours, the intracellular content of Cu2+ decreased from (80.94 +/- 34.76) ng/mg to (46.90 +/- 22.14) ng/mg, P < 0.01, while that of Zn2+ increased from (140.43 +/- 33.81) ng/mg to (151.43 +/- 37.83) ng/mg, P < 0.01. CONCLUSION:GDD is quite effective in removing the intracellular copper and increasing the intracellular zinc.