H Chen1, X Wu, F Guan. 1. First Affiliated Hospital, Dalian Medical University, Liaoning (116011).
Abstract
OBJECTIVE: To explore the protective effects of Tongli Gongxia (TLGX) herbs on gut barrier with multiple organ dysfunction syndrome (MODS). METHODS: The MODS models were induced by intraperitoneal injection of Zymosan A, and Dachengqi decoction (DCQD) was used in treating MODS models. The levels of endotoxin in the peripheral and portal blood, and the contents of diamine oxidase (DAO), xanthine oxidase (XOD), malondialdehyde (MDA), reduced glutathione (GSH) and tumor necrosis factor (TNF), the permeability of intestinal mucosa and enterocytes, and the histopathologic changes were observed. RESULTS: The gut barrier function in MODS rats were severely damaged. DCQD could significantly reduce the levels of endotoxin in the blood (P < 0.01), the contents of XOD, MDA and TNF in the blood and intestinal tissue were lowered (P < 0.01), but the levels of GSH in blood and intestinal tissue were raised (P < 0.05). Levels of DAO in the intestinal tissue was markedly increased (P < 0.01). DCQD could reduce the permeability of intestinal mucosa and enterocytes, and attenuate the histopathologic changes of intestinal mucosa in MODS rats. CONCLUSION: TLGX herbs have apparently protective effects on the gut barrier in MODS rats.
OBJECTIVE: To explore the protective effects of Tongli Gongxia (TLGX) herbs on gut barrier with multiple organ dysfunction syndrome (MODS). METHODS: The MODS models were induced by intraperitoneal injection of Zymosan A, and Dachengqi decoction (DCQD) was used in treating MODS models. The levels of endotoxin in the peripheral and portal blood, and the contents of diamine oxidase (DAO), xanthine oxidase (XOD), malondialdehyde (MDA), reduced glutathione (GSH) and tumor necrosis factor (TNF), the permeability of intestinal mucosa and enterocytes, and the histopathologic changes were observed. RESULTS: The gut barrier function in MODS rats were severely damaged. DCQD could significantly reduce the levels of endotoxin in the blood (P < 0.01), the contents of XOD, MDA and TNF in the blood and intestinal tissue were lowered (P < 0.01), but the levels of GSH in blood and intestinal tissue were raised (P < 0.05). Levels of DAO in the intestinal tissue was markedly increased (P < 0.01). DCQD could reduce the permeability of intestinal mucosa and enterocytes, and attenuate the histopathologic changes of intestinal mucosa in MODS rats. CONCLUSION: TLGX herbs have apparently protective effects on the gut barrier in MODS rats.