F Li1, C Niu, B Ye. 1. Department of Cell Biology, Capital University of Medical Sciences, Beijing 100054, China.
Abstract
OBJECTIVE: To detect malaria DNA in mosquitoes. METHODS: A nested polymerase chain reaction (nested PCR) procedure which amplifies a 121 bp DNA of a SSUrRNA gene specific to Plasmodium vivax was used. RESULTS: In laboratory-infected mosquitoes, nested PCR could detect as few as 3 sporozoites or 1 infected mosquito mixed in a group of 99 normal ones. Furthermore, no specific 121 bp band was seen with DNA templates from other malaria parasites or negative mosquitoes. CONCLUSION: Sensitivity and specificity obtained indicated an advantage of the nested PCR over DNA probes or direct PCR for the detection of Plasmodium vivax sporozoites in mosquitoes with low-grade parasitic infections.
OBJECTIVE: To detect malaria DNA in mosquitoes. METHODS: A nested polymerase chain reaction (nested PCR) procedure which amplifies a 121 bp DNA of a SSUrRNA gene specific to Plasmodium vivax was used. RESULTS: In laboratory-infected mosquitoes, nested PCR could detect as few as 3 sporozoites or 1 infected mosquito mixed in a group of 99 normal ones. Furthermore, no specific 121 bp band was seen with DNA templates from other malaria parasites or negative mosquitoes. CONCLUSION: Sensitivity and specificity obtained indicated an advantage of the nested PCR over DNA probes or direct PCR for the detection of Plasmodium vivax sporozoites in mosquitoes with low-grade parasitic infections.
Authors: Desmond H Foley; Genelle Harrison; Jittawadee R Murphy; Megan Dowler; Leopoldo M Rueda; Richard C Wilkerson Journal: Malar J Date: 2012-05-02 Impact factor: 2.979
Authors: Diego F Echeverry; Nicholas A Deason; Victoria Makuru; Jenna Davidson; Honglin Xiao; Julie Niedbalski; Xiaoyu Yu; Jennifer C Stevenson; Hugo Bugoro; Allan Aparaimo; Hedrick Reuben; Robert Cooper; Thomas R Burkot; Tanya L Russell; Frank H Collins; Neil F Lobo Journal: Malar J Date: 2017-05-31 Impact factor: 2.979