Literature DB >> 117795

Increasing the resolution of polyacrylamide gel electrophoresis by varying the degree of gel crosslinking.

G Johnson.   

Abstract

Resolution of polyacrylamide gel electrophoresis may be substantially improved by taking advantage of the gel sieving effects of varying concentrations of bisacrylamide crosslinker. A dilution procedure is described which permits simultaneous variation of both total acrylamide concentration and percent crosslinking within a single linear regression alaysis.

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Year:  1979        PMID： 117795     DOI： 10.1007/bf00498886

Source DB:  PubMed          Journal:  Biochem Genet        ISSN： 0006-2928            Impact factor:   1.890


  18 in total

1.  Use of internal standards in electrophoretic surveys of enzyme polymorphism.

Authors:  G B Johnson
Journal:  Biochem Genet       Date:  1975-12       Impact factor: 1.890

2.  Estimation of molecular weights of small proteins on polyacrylamide gel electrophoresis.

Authors:  A Gonenne; J Lebowitz
Journal:  Anal Biochem       Date:  1975-04       Impact factor: 3.365

3.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

4.  Structural flexibility of isozyme variants: genetic variants in Drosophila disguised by cofactor and subunit binding.

Authors:  G B Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

5.  Multiple forms of drosophila alcohol dehydrogenase. 3. Conversion of one form to another by nicotinamide adenine dinucleotide or acetone.

Authors:  K B Jacobson; J B Murphy; J A Knopp; J R Ortiz
Journal:  Arch Biochem Biophys       Date:  1972-03       Impact factor: 4.013

6.  Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.

Authors:  J L Hedrick; A J Smith
Journal:  Arch Biochem Biophys       Date:  1968-07       Impact factor: 4.013

7.  Estimation of molecular radius, free mobility, and valence using polyacylamide gel electrophoresis.

Authors:  D Rodbard; A Chrambach
Journal:  Anal Biochem       Date:  1971-03       Impact factor: 3.365

8.  [The molecular sieving effect in polyacrylamide gel electrophoresis as a function of molecular size, gel concentration and gel cross linkage, and its use in the determination of molecular weights].

Authors:  W Thorun
Journal:  Z Klin Chem Klin Biochem       Date:  1971-01

9.  Post-Translational Modification as a Potential Explanation of High Levels of Enzyme Polymorphism: Xanthine Dehydrogenase and Aldehyde Oxidase in DROSOPHILA MELANOGASTER.

Authors:  V Finnerty; G Johnson
Journal:  Genetics       Date:  1979-04       Impact factor: 4.562

10.  Evaluation of the Stepwise Mutation Model of Electrophoretic Mobility: Comparison of the Gel Sieving Behavior of Alleles at the Esterase-5 Locus of DROSOPHILA PSEUDOOBSCURA.

Authors:  G B Johnson
Journal:  Genetics       Date:  1977-09       Impact factor: 4.562
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  4 in total

1.  Purification and characterization of carboxylesterases from rat lung.

Authors:  R Gaustad; K Sletten; D Løvhaug; F Fonnum
Journal:  Biochem J       Date:  1991-03-15       Impact factor: 3.857

2.  The aldox-2 locus of Drosophila melanogaster also affects sulfite oxidase and molybdenum metabolism.

Authors:  M M Bentley; R G Meidinger; A C Braaten
Journal:  Biochem Genet       Date:  1989-02       Impact factor: 1.890

3.  Molecular charge and electrophoretic mobility in cetacean myoglobins of known sequence.

Authors:  T McLellan
Journal:  Biochem Genet       Date:  1984-02       Impact factor: 1.890

4.  Microchamber integration unifies distinct separation modes for two-dimensional electrophoresis.

Authors:  Augusto M Tentori; Alex J Hughes; Amy E Herr
Journal:  Anal Chem       Date:  2013-04-24       Impact factor: 6.986
  4 in total

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