Literature DB >> 11777856

Dengue virus replicative intermediate RNA detection by reverse transcription-PCR.

Gilberto Vaughan1, Hiram Olivera, Leopoldo Santos-Argumedo, Abraham Landa, Baltasar Briseño, Alejandro Escobar-Gutiérrez.   

Abstract

Dengue virus replication involves synthesis of a replicative intermediate RNA (RI-RNA), whose presence reveals an actual infection. We report on a simple and rapid reverse transcription-PCR for the detection of viral RI-RNA in infected cells. The product is demonstrated at 20 min postinfection. This method is useful for the study of virus-cell tropism.

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Year:  2002        PMID： 11777856      PMCID： PMC119872          DOI： 10.1128/cdli.9.1.198-200.2002

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  7 in total

1.  Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.

Authors:  R S Lanciotti; C H Calisher; D J Gubler; G J Chang; A V Vorndam
Journal:  J Clin Microbiol       Date:  1992-03       Impact factor: 5.948

Review 2.  Flavivirus genome organization, expression, and replication.

Authors:  T J Chambers; C S Hahn; R Galler; C M Rice
Journal:  Annu Rev Microbiol       Date:  1990       Impact factor: 15.500

3.  Comparison of various methods of detection of different forms of dengue virus type 2 RNA in cultured cells.

Authors:  H S Liu; Y L Lin; C C Chen
Journal:  Acta Virol       Date:  1997-12       Impact factor: 1.162

4.  Replication strategy of Kunjin virus: evidence for recycling role of replicative form RNA as template in semiconservative and asymmetric replication.

Authors:  P W Chu; E G Westaway
Journal:  Virology       Date:  1985-01-15       Impact factor: 3.616

5.  An intermediate in the synthesis of poliovirus RNA.

Authors:  D Baltimore; M Girard
Journal:  Proc Natl Acad Sci U S A       Date:  1966-08       Impact factor: 11.205

6.  Identification and characterization of type 2 dengue virus replicative intermediate and replicative form RNAs.

Authors:  G R Cleaves; T E Ryan; R W Schlesinger
Journal:  Virology       Date:  1981-05       Impact factor: 3.616

7.  Hepatitis C virus infection in hepatocellular carcinoma. Detection of plus-strand and minus-strand viral RNA.

Authors:  S Takeda; M Shibata; T Morishima; A Harada; A Nakao; H Takagi; Y Nagai
Journal:  Cancer       Date:  1992-11-01       Impact factor: 6.860
  7 in total
  2 in total

1.  Zika virus disrupts gene expression in human myoblasts and myotubes: Relationship with susceptibility to infection.

Authors:  Ingo Riederer; Daniella Arêas Mendes-da-Cruz; Guilherme Cordenonsi da Fonseca; Mariela Natacha González; Otavio Brustolini; Cássia Rocha; Guilherme Loss; Joseane Biso de Carvalho; Mariane Talon Menezes; Lidiane Menezes Souza Raphael; Alexandra Gerber; Myrna Cristina Bonaldo; Gillian Butler-Browne; Vincent Mouly; Vinicius Cotta-de-Almeida; Wilson Savino; Ana Tereza Ribeiro de Vasconcelos
Journal:  PLoS Negl Trop Dis       Date:  2022-02-16

2.  Evidence of vertical transmission of Zika virus in field-collected eggs of Aedes aegypti in the Brazilian Amazon.

Authors:  Cristiano Fernandes da Costa; Arlesson Viana da Silva; Valdinete Alves do Nascimento; Victor Costa de Souza; Dana Cristina da Silva Monteiro; Wagner Cosme Morhy Terrazas; Ricardo Augusto Dos Passos; Suzete Nascimento; José Bento Pereira Lima; Felipe Gomes Naveca
Journal:  PLoS Negl Trop Dis       Date:  2018-07-16
  2 in total

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