C Xing1, J Chen, H Xu. 1. Department of Oncology, First Affiliated Hospital, China Medical University, Shenyang 110001.
Abstract
OBJECTIVE: To study the change and significance of intracellular-free calcium levels ([Ca2+]i) and inositol 1, 4, 5-trisphosphate (IP3) levels in the process of gastric carcinoma cell apoptosis induced by cisplatin. METHODS: Apoptosis induced by cisplatin in gastric carcinoma BGC823 cell was investigated by light and electronmicroscopy, agarose gel electrophoresis and flow cytometry. [Ca2+]i was determined by Fura-2 fluorescin load technic, IP3 was determined by competitive protein binding method. RESULTS: When BGC823 cells were treated with cisplatin (2 micrograms/ml) for 24 hours changes appeared typical of apoptosis. [Ca2+]i and IP3 were significantly increased, especially at the initial stage of apoptosis. However, from 2 to 24 hours after cisplatin treatment, IP3 levels progressively decreased, being significantly lower than those of the untreated controls. CONCLUSION: In the process of cisplatin induced apoptosis of gastric carcinoma cells, [Ca2+]i and IP3 levels are up-regulated at the very initial stage but down-regulated from 2 hours afterwards.
OBJECTIVE: To study the change and significance of intracellular-free calcium levels ([Ca2+]i) and inositol 1, 4, 5-trisphosphate (IP3) levels in the process of gastric carcinoma cell apoptosis induced by cisplatin. METHODS: Apoptosis induced by cisplatin in gastric carcinoma BGC823 cell was investigated by light and electronmicroscopy, agarose gel electrophoresis and flow cytometry. [Ca2+]i was determined by Fura-2 fluorescin load technic, IP3 was determined by competitive protein binding method. RESULTS: When BGC823 cells were treated with cisplatin (2 micrograms/ml) for 24 hours changes appeared typical of apoptosis. [Ca2+]i and IP3 were significantly increased, especially at the initial stage of apoptosis. However, from 2 to 24 hours after cisplatin treatment, IP3 levels progressively decreased, being significantly lower than those of the untreated controls. CONCLUSION: In the process of cisplatin induced apoptosis of gastric carcinoma cells, [Ca2+]i and IP3 levels are up-regulated at the very initial stage but down-regulated from 2 hours afterwards.
Authors: Markus Leo; Linda-Isabell Schmitt; Patricia Küsterarent; Andrea Kutritz; Tienush Rassaf; Christoph Kleinschnitz; Ulrike B Hendgen-Cotta; Tim Hagenacker Journal: Int J Mol Sci Date: 2020-11-16 Impact factor: 5.923