X Zhang1, Y Zhou, B Xu. 1. Department of Pharmacology, Shanghai Institute of Material Medical, Chinese Academy of Sciences, Shanghai 200031, China.
Abstract
OBJECTIVE: To study the differentiation-inducing effect of hydroxycamptothecin (HCPT) on hepatoma Hep G2 cells. METHODS: 3-[4, 5-dimethylthial-2-yl]-3, 5-diphenyl-tetrazolinium bromide (MTT) colorimetric method was used for determining HepG2 cells viability. Alphafetoprotein (AFP) and albumin (ALB) levels were determined with Elisa Kits. RESULTS: The AFP secretion of HCPT-treated cells was reduced by 44.0%-49.2% while the ALB level was increased markedly. CONCLUSION: Hydroxycamptothecin can induce human hepatoma Hep G2 cells to differentiation.
OBJECTIVE: To study the differentiation-inducing effect of hydroxycamptothecin (HCPT) on hepatoma Hep G2 cells. METHODS:3-[4, 5-dimethylthial-2-yl]-3, 5-diphenyl-tetrazolinium bromide (MTT) colorimetric method was used for determining HepG2 cells viability. Alphafetoprotein (AFP) and albumin (ALB) levels were determined with Elisa Kits. RESULTS: The AFP secretion of HCPT-treated cells was reduced by 44.0%-49.2% while the ALB level was increased markedly. CONCLUSION:Hydroxycamptothecin can induce humanhepatoma Hep G2 cells to differentiation.