BACKGROUND: The genes cagA and vacA encode H pylori virulence factors. AIM: To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms. MATERIAL AND METHODS: We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture for H pylori. Detection of cagA and vacA genotyping was done using polymerase chain reactions. RESULTS: The gene cagA was detected in 19 samples (38%). Signal sequences s1 and s2 of vacA gene were detected in 16 samples each (32%). There was simultaneous amplification of s1 and s2 in 6 samples and they were not detected in 9 samples. The middle region of vacA was m1 in 9 samples, m2 in 29 samples and there was simultaneous amplification of m1 and m2 in 12 samples. In 16 samples (32%), more than one type of signal sequence or medial region was detected. Of those patients in whom vacA was the only genotype detected, 15 were s2/m2, 7 were s1/m1, 4 were s1/m2 and 1 was s2/m1. CONCLUSIONS: In these patients, the infection with cagA- H pylori strains, predominates, the prevalence of infection with s1 or s2 strains is similar and the predominant medial region is m2.
BACKGROUND: The genes cagA and vacA encode H pylori virulence factors. AIM: To genotype these genes in H pylori strains isolated from patients with upper gastrointestinal symptoms. MATERIAL AND METHODS: We studied 50 patients who underwent an upper gastrointestinal endoscopy, with positive culture for H pylori. Detection of cagA and vacA genotyping was done using polymerase chain reactions. RESULTS: The gene cagA was detected in 19 samples (38%). Signal sequences s1 and s2 of vacA gene were detected in 16 samples each (32%). There was simultaneous amplification of s1 and s2 in 6 samples and they were not detected in 9 samples. The middle region of vacA was m1 in 9 samples, m2 in 29 samples and there was simultaneous amplification of m1 and m2 in 12 samples. In 16 samples (32%), more than one type of signal sequence or medial region was detected. Of those patients in whom vacA was the only genotype detected, 15 were s2/m2, 7 were s1/m1, 4 were s1/m2 and 1 was s2/m1. CONCLUSIONS: In these patients, the infection with cagA- H pylori strains, predominates, the prevalence of infection with s1 or s2 strains is similar and the predominant medial region is m2.
Authors: S Alaoui Boukhris; S A Boukhris; D-a Benajah; K El Rhazi; S A Ibrahimi; C Nejjari; A Amarti; M Mahmoud; M El Abkari; A Souleimani; B Bennani Journal: Eur J Clin Microbiol Infect Dis Date: 2011-12-11 Impact factor: 3.267
Authors: M I Diaz; A Valdivia; P Martinez; J L Palacios; P Harris; J Novales; E Garrido; D Valderrama; C Shilling; A Kirberg; E Hebel; J Fierro; R Bravo; F Siegel; G Leon; G Klapp; A Venegas Journal: World J Gastroenterol Date: 2005-10-28 Impact factor: 5.742
Authors: Petr Lukeš; Emil Pavlík; Bela Potuznikova; Eva Nartova; Eva Foltynova; Jan Plzak; Rami Katra; Ivan Sterzl; Jirina Bartunkova; Jan Betka; Jaromir Astl Journal: Eur Arch Otorhinolaryngol Date: 2013-06-07 Impact factor: 2.503