Literature DB >> 11772657

Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis.

Carrie Reed1, Gregory D Sturbaum, Paul J Hoover, Charles R Sterling.   

Abstract

Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.

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Year:  2002        PMID： 11772657      PMCID： PMC126566          DOI： 10.1128/AEM.68.1.427-429.2002

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  18 in total

1.  Waterborne cryptosporidiosis: current status.

Authors:  H V Smith; J B Rose
Journal:  Parasitol Today       Date:  1998-01

2.  Species-specific, nested PCR-restriction fragment length polymorphism detection of single Cryptosporidium parvum oocysts.

Authors:  G D Sturbaum; C Reed; P J Hoover; B H Jost; M M Marshall; C R Sterling
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

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5.  A new restriction fragment length polymorphism from Cryptosporidium parvum identifies genetically heterogeneous parasite populations and genotypic changes following transmission from bovine to human hosts.

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Authors:  S Patel; S Pedraza-Díaz; J McLauchlin; D P Casemore
Journal:  Commun Dis Public Health       Date:  1998-12
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6.  Intra-isolate variation of Cryptosporidium parvum small subunit ribosomal RNA genes from human hosts in England.

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7.  An evaluation of primers amplifying DNA targets for the detection of Cryptosporidium spp. using C. parvum HNJ-1 Japanese isolate in water samples.

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9.  Mixed Cryptosporidium infections and HIV.

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