Literature DB >> 11772653

Cloning and characterization of the crystal protein-encoding gene of Bacillus thuringiensis subsp. yunnanensis.

Periasamy Balasubramanian1, R Jayakumar, Prashant Shambharkar, N Unnamalai, S Karutha Pandian, N Selvamuthu Kumaraswami, R Ilangovan, Vaithilingam Sekar.   

Abstract

Molecular cloning and characterization of a novel cry gene, cry32Aa, of Bacillus thuringiensis subsp. yunnanensis was carried out. The Cry32Aa protein was predicted to have a molecular mass of 139.2 kDa and was found to have an unusual 42-amino-acid-long tail at the C terminus. The cry32Aa gene was localized on the 103-MDa plasmid of the organism. Bioassays showed no toxicity against several moths and mosquitoes. However, it exhibited weak toxicity against larvae of the diamondback moth, Plutella xylostella.

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Year:  2002        PMID： 11772653      PMCID： PMC126593          DOI： 10.1128/AEM.68.1.408-411.2002

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  18 in total

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Journal:  Nucleic Acids Res       Date:  1994-11-11       Impact factor: 16.971

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Journal:  J Biol Chem       Date:  1983-02-10       Impact factor: 5.157
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  5 in total

1.  Mining new crystal protein genes from Bacillus thuringiensis on the basis of mixed plasmid-enriched genome sequencing and a computational pipeline.

Authors:  Weixing Ye; Lei Zhu; Yingying Liu; Neil Crickmore; Donghai Peng; Lifang Ruan; Ming Sun
Journal:  Appl Environ Microbiol       Date:  2012-04-27       Impact factor: 4.792

2.  New strategy for isolating novel nematicidal crystal protein genes from Bacillus thuringiensis strain YBT-1518.

Authors:  Suxia Guo; Mei Liu; Donghai Peng; Sisi Ji; Pengxia Wang; Ziniu Yu; Ming Sun
Journal:  Appl Environ Microbiol       Date:  2008-09-26       Impact factor: 4.792

3.  Molecular characterization and genetic diversity of insecticidal crystal protein genes in native Bacillus thuringiensis isolates.

Authors:  H M Mahadeva Swamy; R Asokan; Riaz Mahmood; S N Nagesha
Journal:  Curr Microbiol       Date:  2012-12-04       Impact factor: 2.188

4.  Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae).

Authors:  Geysla da Costa Fernandes; Dalton Kaynnan de Prado Costa; Nayanne Santos de Oliveira; Emanuelle Cristine Pereira de Sousa; Déborah Heloísa Bittencourt Machado; Ricardo Antonio Polanczyk; Herbert Álvaro Abreu de Siqueira; Maria Cleoneide da Silva
Journal:  Sci Rep       Date:  2022-08-24       Impact factor: 4.996

5.  A Genomic and Proteomic Approach to Identify and Quantify the Expressed Bacillus thuringiensis Proteins in the Supernatant and Parasporal Crystal.

Authors:  Joaquín Gomis-Cebolla; Ana Paula Scaramal Ricietto; Juan Ferré
Journal:  Toxins (Basel)       Date:  2018-05-10       Impact factor: 4.546
  5 in total

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