Literature DB >> 11772055

Internal derivatization of oligonucleotides with selenium for X-ray crystallography using MAD.

Quan Du1, Nicolas Carrasco, Marianna Teplova, Christopher J Wilds, Martin Egli, Zhen Huang.   

Abstract

We have developed a route for the synthesis of 2'-selenium uridine analogues and oligonucleotides containing selenium labels, and have demonstrated for the first time a new strategy to covalently derivatize nucleotides with selenium for phase and structure determination in X-ray crystallography.

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Year:  2002        PMID： 11772055     DOI： 10.1021/ja0171097

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  24 in total

1.  Synthesis of the tellurium-derivatized phosphoramidites and their incorporation into DNA oligonucleotides.

Authors:  Sibo Jiang; Jia Sheng; Zhen Huang
Journal:  Curr Protoc Nucleic Acid Chem       Date:  2011-12

2.  Structural basis for Diels-Alder ribozyme-catalyzed carbon-carbon bond formation.

Authors:  Alexander Serganov; Sonja Keiper; Lucy Malinina; Valentina Tereshko; Eugene Skripkin; Claudia Höbartner; Anna Polonskaia; Anh Tuân Phan; Richard Wombacher; Ronald Micura; Zbigniew Dauter; Andres Jäschke; Dinshaw J Patel
Journal:  Nat Struct Mol Biol       Date:  2005-02-20       Impact factor: 15.369

3.  Efficient substrate cleavage catalyzed by hammerhead ribozymes derivatized with selenium for X-ray crystallography.

Authors:  Gary Brandt; Nicolas Carrasco; Zhen Huang
Journal:  Biochemistry       Date:  2006-07-25       Impact factor: 3.162

4.  Synthesis of a 2'-Se-thymidine phosphoramidite and its incorporation into oligonucleotides for crystal structure study.

Authors:  Jia Sheng; Jiansheng Jiang; Jozef Salon; Zhen Huang
Journal:  Org Lett       Date:  2007-01-31       Impact factor: 6.005

5.  Synthesis of a 2'-Se-uridine phosphoramidite and its incorporation into oligonucleotides for structural study.

Authors:  Jozef Salon; Guexiong Chen; Yoani Portilla; Markus W Germann; Zhen Huang
Journal:  Org Lett       Date:  2005-12-08       Impact factor: 6.005

6.  A fast selenium derivatization strategy for crystallization and phasing of RNA structures.

Authors:  Vincent Olieric; Ulrike Rieder; Kathrin Lang; Alexander Serganov; Clemens Schulze-Briese; Ronald Micura; Philippe Dumas; Eric Ennifar
Journal:  RNA       Date:  2009-02-18       Impact factor: 4.942

7.  Mild detritylation of nucleic acid hydroxyl groups by warming up.

Authors:  Jozef Salon; Bo Zhang; Zhen Huang
Journal:  Nucleosides Nucleotides Nucleic Acids       Date:  2011-04       Impact factor: 1.381

8.  The importance of fitting in: conformational preference of selenium 2' modifications in nucleosides and helical structures.

Authors:  R Adam Thompson; Alexander M Spring; Jia Sheng; Zhen Huang; Markus W Germann
Journal:  J Biomol Struct Dyn       Date:  2014-02-21

9.  Structure of a TrmA-RNA complex: A consensus RNA fold contributes to substrate selectivity and catalysis in m5U methyltransferases.

Authors:  Akram Alian; Tom T Lee; Sarah L Griner; Robert M Stroud; Janet Finer-Moore
Journal:  Proc Natl Acad Sci U S A       Date:  2008-05-01       Impact factor: 11.205

Review 10.  Crystallographic studies of chemically modified nucleic acids: a backward glance.

Authors:  Martin Egli; Pradeep S Pallan
Journal:  Chem Biodivers       Date:  2010-01       Impact factor: 2.408
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