| Literature DB >> 11763129 |
O Kirchner1, K H Gartemann, E M Zellermann, R Eichenlaub, A Burger.
Abstract
A transposon mutagenesis system for Clavibacter michiganensis subsp. michiganensis was developed based on antibiotic resistance transposons that were derived from the insertion element IS1409 from Arthrobacter sp. strain TM1 NCIB12013. As a prerequisite, the electroporation efficiency was optimized by using unmethylated DNA and treatment of the cells with glycine such that about 5 x 10(6) transformants per microg of DNA were generally obtained. Electroporation of C. michiganensis subsp. michiganensis with a suicide vector carrying transposon Tn1409C resulted in approximately 1 x 10(3) transposon mutants per pg of DNA and thus is suitable for saturation mutagenesis. Analysis of Tn1409C insertion sites suggests a random mode of transposition. Transposition of Tn1409C was also demonstrated for other subspecies of C. michiganensis.Entities:
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Year: 2001 PMID： 11763129 DOI： 10.1094/MPMI.2001.14.11.1312
Source DB: PubMed Journal: Mol Plant Microbe Interact ISSN： 0894-0282 Impact factor: 4.171