Literature DB >> 1176156

Establishment of a cell line (SH-4) from pleural effusion of a patient with melanoma.

G Seman, S J Hunter, J M Lukeman, L Dmochowski.   

Abstract

A continuous tissue culture line (SH-4) derived from pleural effusion cells of a patient with metastatic melanoma is described. The cells became established after five passages lasting a period of 5 months of slow growth. Doubling time of the continuous culture was 20 hr in passage 51. The cell line is now in passage 60. The cells of all passages examined appear spindle-shaped or oligodendritic as seen by light microscopy. Pigment deposition in the cells is light and incomplete, but more marked than in cells of the original pleural effusion as shown by electron microscopy. Chromosome complement shows a single peak with a modal number of 51.

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Year:  1975        PMID： 1176156     DOI： 10.1007/bf02616335

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  15 in total

1.  Antinucleolar antibodies in the sera of patients with malignant melanoma.

Authors:  C M McBride; J M Bowen; L L Dmochowski
Journal:  Surg Forum       Date:  1972

2.  Tissue culture studies on pleural effusions from breast carcinoma patients.

Authors:  R Cailleau; B Mackay; R K Young; W J Reeves
Journal:  Cancer Res       Date:  1974-04       Impact factor: 12.701

3.  Demonstration of cell-mediated immunity to human neoplasms of various histological types.

Authors:  I Hellström; K E Hellström; H O Sjögren; G A Warner
Journal:  Int J Cancer       Date:  1971-01-15       Impact factor: 7.396

4.  Demonstration of antibodies against human malignant melanoma by immunofluorescence.

Authors:  D L Morton; R A Malmgren; E C Holmes; A S Ketcham
Journal:  Surgery       Date:  1968-07       Impact factor: 3.982

5.  Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma.

Authors:  R K Young; R M Cailleau; B Mackay; W J Reeves
Journal:  In Vitro       Date:  1974 Jan-Feb

6.  Stable chromosome changes in human malignant melanoma.

Authors:  T R Chen; M W Shaw
Journal:  Cancer Res       Date:  1973-09       Impact factor: 12.701

7.  Suspension culture of a pigment-producing cell line derived from a human malignant melanoma.

Authors:  H F Oettgen; T Aoki; L J Old; E A Boyse; E de Harven; G M Mills
Journal:  J Natl Cancer Inst       Date:  1968-10       Impact factor: 13.506

8.  Ultrastructure of human melanoma in cell culture. Electron microscopy studies.

Authors:  S Toshima; G E Moore; A A Sandberg
Journal:  Cancer       Date:  1968-02       Impact factor: 6.860

9.  In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.

Authors:  D J Giard; S A Aaronson; G J Todaro; P Arnstein; J H Kersey; H Dosik; W P Parks
Journal:  J Natl Cancer Inst       Date:  1973-11       Impact factor: 13.506

10.  Studies on the relationship of viruses to the origin of human breast cancer. II. Viruslike particles in human breast tumors.

Authors:  G Seman; B Myers; W C Williams; H S Gallager; L Dmochowski
Journal:  Tex Rep Biol Med       Date:  1969
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  4 in total

1.  Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule.

Authors:  R L Ceriani; K Thompson; J A Peterson; S Abraham
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

2.  Quantitation of human mammary epithelial antigens in cells cultured from normal and cancerous breast tissues.

Authors:  M Sasaki; J A Peterson; R L Ceriani
Journal:  In Vitro       Date:  1981-02

3.  Role of G0-G1 arrest in the inhibition of tumor cell growth by interferon.

Authors:  A A Creasey; J C Bartholomew; T C Merigan
Journal:  Proc Natl Acad Sci U S A       Date:  1980-03       Impact factor: 11.205

4.  Biological properties of human melanoma cells in culture.

Authors:  A A Creasey; H S Smith; A J Hackett; K Fukuyama; W L Epstein; S H Madin
Journal:  In Vitro       Date:  1979-05
  4 in total

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