[Microeletrophoresis in continuous polyacrylamide gel gradients. IV. The effect of reducing agents on the electrophoresis of proteins in sodium dodecylsulfate buffer systems (author's transl)].

The migration of reducing agents (e.g. 2-mercaptoethanol, dithiothreitol and thioglycolic acid) was analysed in various electrophoretic buffer systems containing sodium dodecylsulfate. It is shown that proteins loaded with dodecylsulfate and previously not reduced can be reduced during the electrophoretic separation.