Free radical-induced protein degradation of erythrocyte membrane is influenced by the localization of radical generation.

We have investigated the role of the localization of free radical generation in erythrocyte membrane proteins degradation. The extracellular radical producer 2,2'-azobis(2-amidinopropane) hydrochloride induced a greater loss of band-3 protein in comparison with spectrin whereas the intracellular radical initiator cysteine induced the reverse effect. However, a large extent of main-chain fragmentation was observed for both proteins under the action of cysteine-derived radicals. The results show that the relative localization of the radical generation has an important influence on the degradation of specific proteins of the erythrocyte membrane.