Literature DB >> 11750830

Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains.

M A Pérez1, F J Gallego, P Hidalgo.   

Abstract

The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles obtained for each of the techniques allowed determination of the degree of polymorphism, genetic diversity and relationships among the strains. Consequently, the discriminatory power and usefulness of the molecular techniques assayed was established. SSR analysis was the most effective method due to its high level of polymorphism, the ease of interpretation of the genetic profiles obtained, and the speed of use.

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Year:  2001        PMID： 11750830     DOI： 10.1111/j.1574-6968.2001.tb10975.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  7 in total

1.  Biochemical and molecular characterization of Saccharomyces cerevisiae strains obtained from sugar-cane juice fermentations and their impact in cachaça production.

Authors:  Valdinéia Aparecida Oliveira; Maristela Araújo Vicente; Luciano Gomes Fietto; Ieso de Miranda Castro; Maurício Xavier Coutrim; Dorit Schüller; Henrique Alves; Margarida Casal; Juliana de Oliveira Santos; Leandro Dias Araújo; Paulo Henrique Alves da Silva; Rogelio Lopes Brandão
Journal:  Appl Environ Microbiol       Date:  2007-12-07       Impact factor: 4.792

2.  Molecular relationships between Saccharomyces cerevisiae strains involved in winemaking from Mendoza, Argentina.

Authors:  Laura Mercado; Sandra Jubany; Carina Gaggero; Ricardo W Masuelli; Mariana Combina
Journal:  Curr Microbiol       Date:  2010-04-21       Impact factor: 2.188

3.  Introducing a Cell-Free Approach for the Identification of Brewing Yeast (Saccharomyces cerevisiae) Strains Using MALDI-TOF MS.

Authors:  Elsa Gorre; Cathy Muste; Kevin G Owens
Journal:  J Am Soc Mass Spectrom       Date:  2018-08-07       Impact factor: 3.109

Review 4.  The Biology of Pichia membranifaciens Killer Toxins.

Authors:  Ignacio Belda; Javier Ruiz; Alejandro Alonso; Domingo Marquina; Antonio Santos
Journal:  Toxins (Basel)       Date:  2017-03-23       Impact factor: 4.546

5.  Comparison of Phylogenetic Tree Topologies for Nitrogen Associated Genes Partially Reconstruct the Evolutionary History of Saccharomyces cerevisiae.

Authors:  Manuel Villalobos-Cid; Francisco Salinas; Eduardo I Kessi-Pérez; Matteo De Chiara; Gianni Liti; Mario Inostroza-Ponta; Claudio Martínez
Journal:  Microorganisms       Date:  2019-12-23

6.  The Geographic Distribution of Saccharomyces cerevisiae Isolates within three Italian Neighboring Winemaking Regions Reveals Strong Differences in Yeast Abundance, Genetic Diversity and Industrial Strain Dissemination.

Authors:  Alessia Viel; Jean-Luc Legras; Chiara Nadai; Milena Carlot; Angiolella Lombardi; Manna Crespan; Daniele Migliaro; Alessio Giacomini; Viviana Corich
Journal:  Front Microbiol       Date:  2017-08-24       Impact factor: 5.640

7.  Development and implementation of multilocus sequence typing to study the diversity of the yeast Kluyveromyces marxianus in Italian cheeses.

Authors:  Fabrizia Tittarelli; Javier A Varela; Loughlin Gethins; Catherine Stanton; R P Ross; Giovanna Suzzi; Luigi Grazia; Rosanna Tofalo; John P Morrissey
Journal:  Microb Genom       Date:  2018-01-18
  7 in total

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