AIM: To study the effects of genistein (GE) and quercetin (QU) on proliferation, collagen synthesis, and procollagen messenger RNA (mRNA) expression of rat hepatic stellate cell line HSC-T6 cells. METHODS: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by [3H]proline incorporation assay. Type I mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: GE (25 - 70 micromol/L) and QU (6.25 - 50 micromol/L) concentration-dependently reduced serum-driven HSC-T6 cell proliferation and collagen synthesis associated with a suppression of type I procollagen mRNA level. CONCLUSION: GE and QU inhibited hepatic stellate cell proliferation and collagen synthesis that might have a protective role against liver fibrosis.
AIM: To study the effects of genistein (GE) and quercetin (QU) on proliferation, collagen synthesis, and procollagen messenger RNA (mRNA) expression of rat hepatic stellate cell line HSC-T6 cells. METHODS: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by [3H]proline incorporation assay. Type I mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: GE (25 - 70 micromol/L) and QU (6.25 - 50 micromol/L) concentration-dependently reduced serum-driven HSC-T6 cell proliferation and collagen synthesis associated with a suppression of type I procollagen mRNA level. CONCLUSION: GE and QU inhibited hepatic stellate cell proliferation and collagen synthesis that might have a protective role against liver fibrosis.
Authors: Nalan Kuzu; Kerem Metin; Adile Ferda Dagli; Fatih Akdemir; Cemal Orhan; Mehmet Yalniz; Ibrahim Hanifi Ozercan; Kazim Sahin; Ibrahim Halil Bahcecioglu Journal: Mediators Inflamm Date: 2007 Impact factor: 4.711