T C Qin1, L Chen, L X Yu, Z L Gu. 1. Department of Pharmacology, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China.
Abstract
AIM: To study the inhibitory effect of quercetin on cultured neonatal rat cardiomyocytes hypertrophy induced by angiotensin II (AngII) and its mechanism. METHODS: DNA, RNA, and protein synthesis were measured by incorporation of [3H]thymidine, [14C]uridine, and [3H]tyrosine, respectively. Protein content were measured by Lowry's method. Protein kinase C (PKC) activity was assayed by incubating PKC with histone IIIS and [gamma-(32)P]ATP. Tyrosine protein kinase (TPK) activity was assayed by incubating TPK with poly glutamate/tyrosine (4:1) and [gamma-(32P]ATP. RESULTS: After treated with AngIIat 10 nmol/L for 24 h, total protein content was greatly increased as compared with untreated group (P < 0.01). Incorporation of [14C]uridine and [3H]tyrosine was also greatly increased (P < 0.01) respectively, while incorporation of [3H]thymidine remained unchanged (P > 0.05). Ang II strongly increased cardiomyocytes PKC and TPK activities at 30 min. Que (1-100 micromol/L) could inhibit the increase of total protein content, incorporation of [14C]uridine and [3H]tyrosine, and PKC and TPK activities induced by Ang II in concentration-dependent manner. CONCLUSION: The inhibitory effect of Que on the cardiomyocytes hypertrophy was related to its inhibitory effect on PKC and TPK.
AIM: To study the inhibitory effect of quercetin on cultured neonatal rat cardiomyocytes hypertrophy induced by angiotensin II (AngII) and its mechanism. METHODS: DNA, RNA, and protein synthesis were measured by incorporation of [3H]thymidine, [14C]uridine, and [3H]tyrosine, respectively. Protein content were measured by Lowry's method. Protein kinase C (PKC) activity was assayed by incubating PKC with histone IIIS and [gamma-(32)P]ATP. Tyrosine protein kinase (TPK) activity was assayed by incubating TPK with poly glutamate/tyrosine (4:1) and [gamma-(32P]ATP. RESULTS: After treated with AngIIat 10 nmol/L for 24 h, total protein content was greatly increased as compared with untreated group (P < 0.01). Incorporation of [14C]uridine and [3H]tyrosine was also greatly increased (P < 0.01) respectively, while incorporation of [3H]thymidine remained unchanged (P > 0.05). Ang II strongly increased cardiomyocytes PKC and TPK activities at 30 min. Que (1-100 micromol/L) could inhibit the increase of total protein content, incorporation of [14C]uridine and [3H]tyrosine, and PKC and TPK activities induced by Ang II in concentration-dependent manner. CONCLUSION: The inhibitory effect of Que on the cardiomyocytes hypertrophy was related to its inhibitory effect on PKC and TPK.
Authors: Elena Ulasova; Jessica Perez; Bradford G Hill; Wayne E Bradley; David W Garber; Aimee Landar; Stephen Barnes; Jeevan Prasain; Dale A Parks; Louis J Dell'Italia; Victor M Darley-Usmar Journal: Redox Biol Date: 2013-07-16 Impact factor: 11.799