X C Zhou1, P Wei, Z Y Hu, F Gao, R J Zhou, Y X Liu. 1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
Abstract
AIM: To investigate role of apoptosis related genes Fas/FasL in azoospermia or oligozoospermia induced by testosterone undecanoate. METHODS: TUNEL was used to detect the apoptotic signal of testicular cells. Immunohistochemistry and Western blot were used to quantitatively or qualitatively analyze the expression of these apoptosis-related proteins. RESULTS: After injection of testosterone undecanoate, both the apoptotic signal in germ cells and the expression of Fas/FasL in testis increased correlatively in a time-dependent manner, reaching a maximum level on day 30. CONCLUSION: Fas system may initiate and regulate the germ cell apoptosis induced by testosterone undecanoate.
AIM: To investigate role of apoptosis related genes Fas/FasL in azoospermia or oligozoospermia induced by testosterone undecanoate. METHODS: TUNEL was used to detect the apoptotic signal of testicular cells. Immunohistochemistry and Western blot were used to quantitatively or qualitatively analyze the expression of these apoptosis-related proteins. RESULTS: After injection of testosterone undecanoate, both the apoptotic signal in germ cells and the expression of Fas/FasL in testis increased correlatively in a time-dependent manner, reaching a maximum level on day 30. CONCLUSION: Fas system may initiate and regulate the germ cell apoptosis induced by testosterone undecanoate.
Authors: Katharina Gegenschatz-Schmid; Gilvydas Verkauskas; Philippe Demougin; Vytautas Bilius; Darius Dasevicius; Michael B Stadler; Faruk Hadziselimovic Journal: Basic Clin Androl Date: 2018-02-09