| Literature DB >> 11741155 |
E Minc1, Y Allory, J C Courvalin, B Buendia.
Abstract
HP1 proteins are conserved non histone chromosomal proteins involved in the epigenetic repression of transcription. Three HP1 proteins, HP1alpha, HP1beta and HP1gamma are expressed in mammalian cells. Polyclonal antibodies directed against peptides specific for HP1alpha and HP1gamma were elicited in rabbits, affinity purified, then used to localize both proteins on spreads of unfixed metaphasic chromosomes. We show here, by conventional and confocal microscopy, that both proteins are localized at centromeres and pericentromeres, and that HP1gamma is also present on euchromatic sites of chromosome arms.Entities:
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Year: 2001 PMID： 11741155 DOI： 10.1007/978-94-010-0330-8_18
Source DB: PubMed Journal: Methods Cell Sci ISSN： 1381-5741