BACKGROUND: Deregulated c-Myc expression and alterations of c-myc oncogene have been reported to play an important role in breast cancer tumorigenesis. We examined the relationship between c-Myc protein level, amplification of c-myc oncogene and commonly used clinical and pathologic factors. METHODS: The studies were conducted on 94 ductal and lobular cancers. Amplification of c-Myc was assessed by the semiquantitative multiplex PCR assay. The amount of c-Myc protein was estimated by the densitometry analysis of Western blots. RESULTS: Amplification of c-Myc was found in 21% of examined cancers. There was no association of c-myc amplification with established risk factors. Overexpression of c-Myc protein without c-myc amplification was associated with negative status of axillary lymph node. The size of lobular carcinoma displaying overexpression of c-Myc and the normal copy number of c-myc gene was significantly smaller than the size of tumor with elevated c-Myc and amplification of c-myc gene (p < 0.01). Within tumors displaying overexpression of c-Myc protein and c-myc gene amplification the size of ductal carcinoma was smaller than the size of lobular carcinoma (p < 0.007). CONCLUSION: Data presented in this study suggest that alterations of c-myc gene and c-Myc protein level might be related to breast cancer progression. The prognostic utility of elevated level of c-Myc protein associated with normal status of c-myc gene for patients with lobular carcinoma requires further studies.
BACKGROUND: Deregulated c-Myc expression and alterations of c-myc oncogene have been reported to play an important role in breast cancer tumorigenesis. We examined the relationship between c-Myc protein level, amplification of c-myc oncogene and commonly used clinical and pathologic factors. METHODS: The studies were conducted on 94 ductal and lobular cancers. Amplification of c-Myc was assessed by the semiquantitative multiplex PCR assay. The amount of c-Myc protein was estimated by the densitometry analysis of Western blots. RESULTS: Amplification of c-Myc was found in 21% of examined cancers. There was no association of c-myc amplification with established risk factors. Overexpression of c-Myc protein without c-myc amplification was associated with negative status of axillary lymph node. The size of lobular carcinoma displaying overexpression of c-Myc and the normal copy number of c-myc gene was significantly smaller than the size of tumor with elevated c-Myc and amplification of c-myc gene (p < 0.01). Within tumors displaying overexpression of c-Myc protein and c-myc gene amplification the size of ductal carcinoma was smaller than the size of lobular carcinoma (p < 0.007). CONCLUSION: Data presented in this study suggest that alterations of c-myc gene and c-Myc protein level might be related to breast cancer progression. The prognostic utility of elevated level of c-Myc protein associated with normal status of c-myc gene for patients with lobular carcinoma requires further studies.
Authors: Nicolaos Palaskas; Steven M Larson; Nikolaus Schultz; Evangelia Komisopoulou; Justin Wong; Dan Rohle; Carl Campos; Nicolas Yannuzzi; Joseph R Osborne; Irina Linkov; Edward R Kastenhuber; Richard Taschereau; Seema B Plaisier; Chris Tran; Adriana Heguy; Hong Wu; Chris Sander; Michael E Phelps; Cameron Brennan; Elisa Port; Jason T Huse; Thomas G Graeber; Ingo K Mellinghoff Journal: Cancer Res Date: 2011-06-06 Impact factor: 12.701
Authors: Carolina Rosselot; Anil Kumar; Jayalakshmi Lakshmipathi; Pili Zhang; Geming Lu; Liora S Katz; Edward V Prochownik; Andrew F Stewart; Luca Lambertini; Donald K Scott; Adolfo Garcia-Ocaña Journal: Diabetes Date: 2019-07-10 Impact factor: 9.461
Authors: Mahnaz Janghorban; Amy S Farrell; Brittany L Allen-Petersen; Carl Pelz; Colin J Daniel; Jessica Oddo; Ellen M Langer; Dale J Christensen; Rosalie C Sears Journal: Proc Natl Acad Sci U S A Date: 2014-06-09 Impact factor: 11.205