L M Katz1, Y Wang, B McMahon, E Richelson. 1. Department of Emergency Medicine, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC 27599, USA. lkatz@med.unc.edu
Abstract
OBJECTIVE: To determine whether the neurotensin analog NT69L, administered systemically, could induce mild brain hypothermia after asphyxial cardiac arrest (ACA) in rats. METHODS: The study design was experimental, blinded, randomized, and approved by the animal use committee. All rats had continuous monitoring of brain temperature and sustained 8 minutes of ACA, resuscitation, and either saline or NT69L intravenously after return of spontaneous circulation (ROSC). Rats surviving 14 days after ACA had a neurological deficit score (NDS) and a Morris Water Maze (MWM) test. RESULTS: Seven of eight rats in each group survived 14 days. Brain temperature was less than 35 degrees C 13.1 +/- 3 minutes (mean +/- standard deviation) after NT69L vs controls that remained 37.5 degrees C at the same ambient temperature (p < 0.05 ANOVA). The NT69L group remained below 35 degrees C for 300 +/- 100 minutes while the controls remained at 37.5 +/- 0.5 degrees C. The NDS in the NT69L rats was 3 +/- 3% vs controls 26 +/- 8% (p < 0.05, Kruskal-Wallis, 0% = normal, 100% = brain dead). The NT69L rats performed better on the MWM vs the controls (22 +/- 8 sec vs 45 +/- 26 sec, respectively, p < 0.05 ANOVA). CONCLUSIONS: NT69L induced rapid and prolonged mild brain hypothermia after ACA in this rat model and reduced neurological deficits.
OBJECTIVE: To determine whether the neurotensin analog NT69L, administered systemically, could induce mild brain hypothermia after asphyxial cardiac arrest (ACA) in rats. METHODS: The study design was experimental, blinded, randomized, and approved by the animal use committee. All rats had continuous monitoring of brain temperature and sustained 8 minutes of ACA, resuscitation, and either saline or NT69L intravenously after return of spontaneous circulation (ROSC). Rats surviving 14 days after ACA had a neurological deficit score (NDS) and a Morris Water Maze (MWM) test. RESULTS: Seven of eight rats in each group survived 14 days. Brain temperature was less than 35 degrees C 13.1 +/- 3 minutes (mean +/- standard deviation) after NT69L vs controls that remained 37.5 degrees C at the same ambient temperature (p < 0.05 ANOVA). The NT69L group remained below 35 degrees C for 300 +/- 100 minutes while the controls remained at 37.5 +/- 0.5 degrees C. The NDS in the NT69L rats was 3 +/- 3% vs controls 26 +/- 8% (p < 0.05, Kruskal-Wallis, 0% = normal, 100% = brain dead). The NT69L rats performed better on the MWM vs the controls (22 +/- 8 sec vs 45 +/- 26 sec, respectively, p < 0.05 ANOVA). CONCLUSIONS: NT69L induced rapid and prolonged mild brain hypothermia after ACA in this rat model and reduced neurological deficits.
Authors: Charles Kim; Denise Barbut; Murk H Heinemann; Gavril Pasternak; Mark I Rosenblatt Journal: Invest Ophthalmol Vis Sci Date: 2014-05-13 Impact factor: 4.799
Authors: Alexis A Topjian; Robert A Berg; Joost J L M Bierens; Christine M Branche; Robert S Clark; Hans Friberg; Cornelia W E Hoedemaekers; Michael Holzer; Laurence M Katz; Johannes T A Knape; Patrick M Kochanek; Vinay Nadkarni; Johannes G van der Hoeven; David S Warner Journal: Neurocrit Care Date: 2012-12 Impact factor: 3.210