Literature DB >> 11728729

Purification of the autotransporter protein Hbp of Escherichia coli.

S J van Dooren1, J R Tame, J Luirink, B Oudega, B R Otto.   

Abstract

The enzyme Hbp (hemoglobin protease) of the pathogenic Escherichia coli strain EB1 has been purified to homogeneity by gel filtration chromatography. The purified protein is capable of binding heme and shows hemoglobin protease activity. Our method of purification is applicable not only to Hbp but also to other autotransporter proteins and will contribute to a better understanding of the function-structure relationship of this family of proteins.

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Year:  2001        PMID： 11728729     DOI： 10.1111/j.1574-6968.2001.tb10938.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  7 in total

1.  A conserved aromatic residue in the autochaperone domain of the autotransporter Hbp is critical for initiation of outer membrane translocation.

Authors:  Zora Soprova; Ana Sauri; Peter van Ulsen; Jeremy R H Tame; Tanneke den Blaauwen; Wouter S P Jong; Joen Luirink
Journal:  J Biol Chem       Date:  2010-10-05       Impact factor: 5.157

Review 2.  Autotransporter passenger proteins: virulence factors with common structural themes.

Authors:  Kaoru Nishimura; Nami Tajima; Young-Ho Yoon; Sam-Yong Park; Jeremy R H Tame
Journal:  J Mol Med (Berl)       Date:  2010-03-09       Impact factor: 4.599

3.  Decoration of outer membrane vesicles with multiple antigens by using an autotransporter approach.

Authors:  Maria H Daleke-Schermerhorn; Tristan Felix; Zora Soprova; Corinne M Ten Hagen-Jongman; David Vikström; Laleh Majlessi; Joep Beskers; Frank Follmann; Karin de Punder; Nicole N van der Wel; Thomas Baumgarten; Thang V Pham; Sander R Piersma; Connie R Jiménez; Peter van Ulsen; Jan-Willem de Gier; Claude Leclerc; Wouter S P Jong; Joen Luirink
Journal:  Appl Environ Microbiol       Date:  2014-07-18       Impact factor: 4.792

4.  Molecular Characterization of the Vacuolating Autotransporter Toxin in Uropathogenic Escherichia coli.

Authors:  Katie B Nichols; Makrina Totsika; Danilo G Moriel; Alvin W Lo; Ji Yang; Daniël J Wurpel; Amanda E Rossiter; Richard A Strugnell; Ian R Henderson; Glen C Ulett; Scott A Beatson; Mark A Schembri
Journal:  J Bacteriol       Date:  2016-04-28       Impact factor: 3.490

5.  A structurally informed autotransporter platform for efficient heterologous protein secretion and display.

Authors:  Wouter S P Jong; Zora Soprova; Karin de Punder; Corinne M ten Hagen-Jongman; Samuel Wagner; David Wickström; Jan-Willem de Gier; Peter Andersen; Nicole N van der Wel; Joen Luirink
Journal:  Microb Cell Fact       Date:  2012-06-18       Impact factor: 5.328

6.  An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines.

Authors:  Wouter S P Jong; Maria H Daleke-Schermerhorn; David Vikström; Corinne M Ten Hagen-Jongman; Karin de Punder; Nicole N van der Wel; Carolien E van de Sandt; Guus F Rimmelzwaan; Frank Follmann; Else Marie Agger; Peter Andersen; Jan-Willem de Gier; Joen Luirink
Journal:  Microb Cell Fact       Date:  2014-11-25       Impact factor: 5.328

7.  Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras.

Authors:  Trang H Phan; Coen Kuijl; Dung T Huynh; Wouter S P Jong; Joen Luirink; Peter van Ulsen
Journal:  Microb Cell Fact       Date:  2021-09-06       Impact factor: 5.328
  7 in total

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