Literature DB >> 11728721

Rapid orientated cloning in a shuttle vector allowing modulated gene expression in Bacillus subtilis.

P Joseph1, J R Fantino, M L Herbaud, F Denizot.   

Abstract

An expression vector for systematic protein overproduction in Bacillus subtilis has been constructed. It derives from pDG148 and combines the main property of this vector, i.e. conditional expression of the gene in response to isopropylbeta-D-thiogalactopyranoside, with (i) rapid orientated cloning by a ligation independent procedure and (ii) a ribosome binding site of high translational efficiency. When used for overproduction of several proteins in B. subtilis, this vector gave good levels of protein synthesis.

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Year:  2001        PMID： 11728721     DOI： 10.1111/j.1574-6968.2001.tb10930.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  33 in total

1.  Differentiation of function among the RsbR paralogs in the general stress response of Bacillus subtilis with regard to light perception.

Authors:  Jeroen B van der Steen; Marcela Avila-Pérez; Doreen Knippert; Angie Vreugdenhil; Pascal van Alphen; Klaas J Hellingwerf
Journal:  J Bacteriol       Date:  2012-01-27       Impact factor: 3.490

2.  Interaction of GapA with HPr and its homologue, Crh: Novel levels of regulation of a key step of glycolysis in Bacillus subtilis?

Authors:  Frédérique Pompeo; Jennifer Luciano; Anne Galinier
Journal:  J Bacteriol       Date:  2006-12-01       Impact factor: 3.490

3.  Resistance to bacitracin in Bacillus subtilis: unexpected requirement of the BceAB ABC transporter in the control of expression of its own structural genes.

Authors:  Remi Bernard; Annick Guiseppi; Marc Chippaux; Maryline Foglino; François Denizot
Journal:  J Bacteriol       Date:  2007-09-28       Impact factor: 3.490

4.  Liberation of zinc-containing L31 (RpmE) from ribosomes by its paralogous gene product, YtiA, in Bacillus subtilis.

Authors:  Genki Akanuma; Hideaki Nanamiya; Yousuke Natori; Naofumi Nomura; Fujio Kawamura
Journal:  J Bacteriol       Date:  2006-04       Impact factor: 3.490

5.  Discovery of a Unique Extracellular Polysaccharide in Members of the Pathogenic Bacillus That Can Co-form with Spores.

Authors:  Zi Li; Soyoun Hwang; Maor Bar-Peled
Journal:  J Biol Chem       Date:  2016-07-11       Impact factor: 5.157

6.  Novel conserved assembly factor of the bacterial flagellum.

Authors:  Björn Titz; Seesandra V Rajagopala; Claudia Ester; Roman Häuser; Peter Uetz
Journal:  J Bacteriol       Date:  2006-08-25       Impact factor: 3.490

7.  The bacteriophage ϕ29 tail possesses a pore-forming loop for cell membrane penetration.

Authors:  Jingwei Xu; Miao Gui; Dianhong Wang; Ye Xiang
Journal:  Nature       Date:  2016-06-15       Impact factor: 49.962

8.  An RNA pyrophosphohydrolase triggers 5'-exonucleolytic degradation of mRNA in Bacillus subtilis.

Authors:  Jamie Richards; Quansheng Liu; Olivier Pellegrini; Helena Celesnik; Shiyi Yao; David H Bechhofer; Ciarán Condon; Joel G Belasco
Journal:  Mol Cell       Date:  2011-09-16       Impact factor: 17.970

9.  Effects of phosphorelay perturbations on architecture, sporulation, and spore resistance in biofilms of Bacillus subtilis.

Authors:  Jan-Willem Veening; Oscar P Kuipers; Stanley Brul; Klaas J Hellingwerf; Remco Kort
Journal:  J Bacteriol       Date:  2006-04       Impact factor: 3.490

10.  Both thiamine uptake and biosynthesis of thiamine precursors are required for intracellular replication of Listeria monocytogenes.

Authors:  Kristina Schauer; Jürgen Stolz; Siegfried Scherer; Thilo M Fuchs
Journal:  J Bacteriol       Date:  2009-01-30       Impact factor: 3.490
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