Literature DB >> 11728714

Effect of environmental and chemotactic stimuli on the activity of the Campylobacter jejuni flaA sigma(28) promoter.

K J Allen1, M W Griffiths.   

Abstract

The effect of environmental and chemotactic stimuli was assessed using a fusion between the Campylobacter jejuni flaA sigma(28) promoter and a promoterless luxCDABE cassette on pRYluxCDABE. Environmental stimuli relevant to the enteric environment were found to modulate flaA promoter activity. pH, bovine bile, deoxycholate, L-fucose and osmolarity up-regulated the flaA promoter while viscosity resulted in down-regulation. C. jejuni chemotactic effectors, including the amino acids aspartate and glutamate and the organic acids citrate, fumarate, alpha-ketoglutarate and succinate up-regulated the flaA promoter. Proline, which does not elicit a chemotactic response, up-regulated the flaA promoter.

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Year:  2001        PMID： 11728714     DOI： 10.1111/j.1574-6968.2001.tb10923.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


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