X Tian1, M Iwatsu, A Kanai. 1. Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.
Abstract
PURPOSE: To investigate the ability of a disposable soft contact lens (1-Day Acuvue) to deliver lomefloxacin, a fluoroquinolones antibiotic, into the cornea and aqueous humor of rabbits. METHODS: Experiments were conducted on adult albino rabbits. 1-Day Acuvue lenses were immersed for 1 hour in commercially-available lomefloxacin eye solution and then placed on the cornea. After 0.5, 2, 4,6, and 8 hours, the animals were killed and the lenses were removed and placed into a 20 mL saline bath. Corneal tissue and aqueous humor samples were also obtained at these times. The release of lomefloxacin from the lenses was calculated by measuring the amount of drug remaining in the lenses after removal from the rabbit eyes. The concentration of lomefloxacin in the cornea and anterior chamber following the wearing of lomefloxacin-loaded lenses was compared with the concentrations following frequent-drop therapy (one drop of lomefloxacin hourly for 8 hours). RESULTS: In vivo, lomefloxacin was released from 1-Day Acuvue lenses gradually over 8 hours. The cornea and aqueous humor levels in the eyes following the wear of lomefloxacin-loaded lenses were significantly higher than those achieved by frequent-drop therapy. The drug levels in the cornea and aqueous humor generally remained above the 90% minimal inhibitory concentration for 8 hours in the 1-Day Acuvue lens group. CONCLUSIONS: 1-Day Acuvue contact lenses soaked in 0.3% lomefloxacin can release sufficient amounts of lomefloxacin and in this study produced higher levels in both the cornea and aqueous humor than that achieved by frequent-drop therapy for up to 8 hours. We conclude that 1-Day Acuvue contact lens can be used as a drug delivery system for lomefloxacin.
PURPOSE: To investigate the ability of a disposable soft contact lens (1-Day Acuvue) to deliver lomefloxacin, a fluoroquinolones antibiotic, into the cornea and aqueous humor of rabbits. METHODS: Experiments were conducted on adult albino rabbits. 1-Day Acuvue lenses were immersed for 1 hour in commercially-available lomefloxacin eye solution and then placed on the cornea. After 0.5, 2, 4,6, and 8 hours, the animals were killed and the lenses were removed and placed into a 20 mL saline bath. Corneal tissue and aqueous humor samples were also obtained at these times. The release of lomefloxacin from the lenses was calculated by measuring the amount of drug remaining in the lenses after removal from the rabbit eyes. The concentration of lomefloxacin in the cornea and anterior chamber following the wearing of lomefloxacin-loaded lenses was compared with the concentrations following frequent-drop therapy (one drop of lomefloxacin hourly for 8 hours). RESULTS: In vivo, lomefloxacin was released from 1-Day Acuvue lenses gradually over 8 hours. The cornea and aqueous humor levels in the eyes following the wear of lomefloxacin-loaded lenses were significantly higher than those achieved by frequent-drop therapy. The drug levels in the cornea and aqueous humor generally remained above the 90% minimal inhibitory concentration for 8 hours in the 1-Day Acuvue lens group. CONCLUSIONS: 1-Day Acuvue contact lenses soaked in 0.3% lomefloxacin can release sufficient amounts of lomefloxacin and in this study produced higher levels in both the cornea and aqueous humor than that achieved by frequent-drop therapy for up to 8 hours. We conclude that 1-Day Acuvue contact lens can be used as a drug delivery system for lomefloxacin.