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Literature DB >> 11725482

Using 2', 7'-dichlorodihydrofluorescein-diacetate to assess polysaccharides as immunomodulating agents.

Abstract

Neutrophils play a key role in the defense against microbial infections. One of their primary weapons to destroy microbes is the production of reactive oxygen species (ROS). This paper shows how 2', 7'-dichlorodihydrofluorescein-diacetate (DCFH-DA) was used to measure the effects of polysaccharides on the production of ROS by polymorphonuclear leukocytes (PMNs). The DCFH-DA method has been designed to provide a highly sensitive, quantifiable, real-time assessment of ROS production by PMNs.

Entities: Chemical Disease

Mesh：

Substances：

Year: 2001 PMID： 11725482 DOI： 10.1385/MB:19:2:125

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

13 in total

1. Soluble beta-glucan polysaccharide binding to the lectin site of neutrophil or natural killer cell complement receptor type 3 (CD11b/CD18) generates a primed state of the receptor capable of mediating cytotoxicity of iC3b-opsonized target cells.

Authors: V Vetvicka; B P Thornton; G D Ross
Journal: J Clin Invest Date: 1996-07-01 Impact factor: 14.808

2. Induction of tumoricidal activity of polymorphonuclear leukocytes by a linear beta-1,3-D-glucan and other immunomodulators in murine cells.

Authors: K Morikawa; R Takeda; M Yamazaki; D Mizuno
Journal: Cancer Res Date: 1985-04 Impact factor: 12.701

3. Lipopolysaccharide isolated from Porphyromonas gingivalis grown in hemin-limited chemostat conditions has a reduced capacity for human neutrophil priming.

Authors: C M Champagne; S C Holt; T E Van Dyke; B J Gordon; L Shapira
Journal: Oral Microbiol Immunol Date: 1996-10

4. Flow cytometric analysis of nitric oxide production in human neutrophils using dichlorofluorescein diacetate in the presence of a calmodulin inhibitor.

Authors: K M Rao; J Padmanabhan; D L Kilby; H J Cohen; M S Currie; J B Weinberg
Journal: J Leukoc Biol Date: 1992-05 Impact factor: 4.962

5. Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).

Authors: I D Trayner; A P Rayner; G E Freeman; F Farzaneh
Journal: J Immunol Methods Date: 1995-10-26 Impact factor: 2.303

6. Further characterization of the neutrophil oxidative burst by flow cytometry.

Authors: J A Smith; M J Weidemann
Journal: J Immunol Methods Date: 1993-06-18 Impact factor: 2.303

7. A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescin-diacetate.

Authors: A R Rosenkranz; S Schmaldienst; K M Stuhlmeier; W Chen; W Knapp; G J Zlabinger
Journal: J Immunol Methods Date: 1992-11-25 Impact factor: 2.303

8. Fluorescent microplate assay for respiratory burst of PMNs challenged in vitro with orthopedic metals.

Authors: G Ciapetti; D Granchi; E Verri; L Savarino; E Cenni; F Savioli; A Pizzoferrato
Journal: J Biomed Mater Res Date: 1998-09-05

9. Influence of polysaccharides on neutrophil function: specific antagonists suggest a model for cooperative saccharide-associated inhibition of immune complex-triggered superoxide production.

Authors: K Zhang; H R Petty
Journal: J Cell Biochem Date: 1994-10 Impact factor: 4.429