Literature DB >> 11724268

Rescue of bovine respiratory syncytial virus from cloned cDNA: entire genome sequence of BRSV strain A51908.

A S Yunus1, S K Khattar, P L Collins, S K Samal.   

Abstract

Infectious bovine respiratory syncytial virus (BRSV) was produced by intracellular co-expression of five plasmid borne cDNAs, each under the control of a T7 RNA polymerase promoter. These separately encoded a full-length, genetically-marked copy of BRSV antigenome along with either BRSV or human respiratory syncytial virus (HRSV) support plasmids, which express N, P, L and M2-1 proteins. HEp2 cells were used in transfection and recombinant vaccinia virus (MVA-T7) provided T7 RNA polymerase to drive the transcription. The recovery of recombinant BRSV (rBRSV) was confirmed by immunological staining of plaques, restriction enzyme digestion and nucleotide sequencing of PCR fragments carrying the genetic markers from the rescued virus. The rBRSV was indistinguishable from its parental wild-type virus in its growth characteristics in cell culture. The present work has completed the entire genome sequence of BRSV strain A51908 (15,140 nt) and has also identified changes in sequence and growth characteristics in cell culture from the original BRSV strain A51908 laboratory isolate.

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Year:  2001        PMID： 11724268     DOI： 10.1023/a:1011888019966

Source DB:  PubMed          Journal:  Virus Genes        ISSN： 0920-8569            Impact factor:   2.198


  23 in total

1.  Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

Authors:  T R Fuerst; E G Niles; F W Studier; B Moss
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

2.  Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5' proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development.

Authors:  P L Collins; M G Hill; E Camargo; H Grosfeld; R M Chanock; B R Murphy
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-05       Impact factor: 11.205

3.  RNA replication by a respiratory syncytial virus RNA analog does not obey the rule of six and retains a nonviral trinucleotide extension at the leader end.

Authors:  S K Samal; P L Collins
Journal:  J Virol       Date:  1996-08       Impact factor: 5.103

4.  Gene junction sequences of bovine respiratory syncytial virus.

Authors:  M Zamora; S K Samal
Journal:  Virus Res       Date:  1992-06       Impact factor: 3.303

5.  Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter.

Authors:  U J Buchholz; S Finke; K K Conzelmann
Journal:  J Virol       Date:  1999-01       Impact factor: 5.103

6.  Nucleotide sequence analysis of a matrix and small hydrophobic protein dicistronic mRNA of bovine respiratory syncytial virus demonstrates extensive sequence divergence of the small hydrophobic protein from that of human respiratory syncytial virus.

Authors:  S K Samal; M Zamora
Journal:  J Gen Virol       Date:  1991-07       Impact factor: 3.891

7.  Sequence analysis of a functional polymerase (L) gene of bovine respiratory syncytial virus: determination of minimal trans-acting requirements for RNA replication.

Authors:  A S Yunus; P L Collins; S K Samal
Journal:  J Gen Virol       Date:  1998-09       Impact factor: 3.891

8.  Sequence analysis of M2 mRNA of bovine respiratory syncytial virus obtained from an F-M2 dicistronic mRNA suggests structural homology with that of human respiratory syncytial virus.

Authors:  M Zamora; S K Samal
Journal:  J Gen Virol       Date:  1992-03       Impact factor: 3.891

9.  Infectious rabies viruses from cloned cDNA.

Authors:  M J Schnell; T Mebatsion; K K Conzelmann
Journal:  EMBO J       Date:  1994-09-15       Impact factor: 11.598

10.  A highly recombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA: generation of a novel copy-back nondefective interfering virus.

Authors:  D Garcin; T Pelet; P Calain; L Roux; J Curran; D Kolakofsky
Journal:  EMBO J       Date:  1995-12-15       Impact factor: 11.598
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4.  Immunofluorescence and molecular diagnosis of bovine respiratory syncytial virus and bovine parainfluenza virus in the naturally infected young cattle and buffaloes from India.

Authors:  Bhupesh Kamdi; Rajendra Singh; Vidya Singh; Shailendra Singh; Pawan Kumar; Karam Pal Singh; Neethu George; Kuldeep Dhama
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  4 in total

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