Genomic organization and expression profile of the parvin family of focal adhesion proteins in mice and humans.

We have characterized the genomic organization and the expression pattern of alpha-, beta- and gamma-parvin, a novel family of focal adhesion proteins, in mice and humans. alpha-Parvin is nearly ubiquitously expressed, beta-parvin is preferentially expressed in heart- and skeletal muscle, and gamma-parvin in lymphoid tissues. Parvins display diverse patterns of developmental regulation. The alpha-form is present throughout mouse development, beta-parvin is gradually upregulated and gamma-parvin is downregulated at embryonic day 11. The human alpha-parvin gene (PARVA), extending over 160 kb, is located on chromosome 11. Both, the human beta-parvin gene (PARVB), which is over 145 kb long, and the gamma-parvin gene (PARVG) of a total length of about 25 kb are positioned on chromosome 22 with PARVG located about 12 kb downstream of the 3' end of PARVB. Multiple tissue array analysis indicates that parvins are expressed at reduced levels in cancer as compared to the corresponding normal tissues. Analysis of ESTs and PCR-amplified fragments reveals alternatively spliced and alternatively polyadenylated gene products. Mammalian parvins are likely to have arisen late in evolution from gene duplication as they share a remarkably similar exon/intron organization, which is different from the organization of the single genes encoding parvin-like proteins in Drosophila and Caenorhabditis.