Literature DB >> 11717514

Purification, crystallization and preliminary X-ray diffraction of a complex between IL-10 and soluble IL-10R1.

K Josephson1, D T McPherson, M R Walter.   

Abstract

A complex between interleukin-10 and the extracellular domain of its high-affinity receptor (sIL-10R1) has been crystallized from polyethylene glycol solutions. Crystals suitable for diffraction analysis required the modification of the NXS/T glycosylation sites on sIL-10R1 by site-directed mutagenesis and inclusion of the detergent cyclohexyl-methyl-beta-D-maltopyranoside in the crystallization experiments. The crystals belong to space group P3(2)12 or its enantimorph P3(1)12, with unit-cell parameters a = b = 46.23, c = 307.78 A, alpha = beta = 90, gamma = 120 degrees, and diffract X-rays to approximately 2.9 A. The IL-10 dimer is positioned on a crystallographic twofold, resulting in one IL-10 chain and one sIL-10R1 chain in the asymmetric unit, which corresponds to a solvent content of approximately 44%.

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Year:  2001        PMID： 11717514     DOI： 10.1107/s0907444901016249

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  9 in total

1.  Purification, crystallization and preliminary X-ray diffraction analysis of the IL-20-IL-20R1-IL-20R2 complex.

Authors:  Naomi J Logsdon; Christopher E Allen; Kanagalaghatta R Rajashankar; Mark R Walter
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-12-24

Review 2.  Structural analysis of cytokines comprising the IL-10 family.

Authors:  Alexander Zdanov
Journal:  Cytokine Growth Factor Rev       Date:  2010-09-16       Impact factor: 7.638

3.  Epstein-Barr virus IL-10 engages IL-10R1 by a two-step mechanism leading to altered signaling properties.

Authors:  Sung Il Yoon; Brandi C Jones; Naomi J Logsdon; Bethany D Harris; Srilalitha Kuruganti; Mark R Walter
Journal:  J Biol Chem       Date:  2012-06-12       Impact factor: 5.157

4.  Crystal structure of human cytomegalovirus IL-10 bound to soluble human IL-10R1.

Authors:  Brandi C Jones; Naomi J Logsdon; Kristopher Josephson; Jennifer Cook; Peter A Barry; Mark R Walter
Journal:  Proc Natl Acad Sci U S A       Date:  2002-07-01       Impact factor: 11.205

5.  Inhibitory mechanism of peptides with a repeating hydrophobic and hydrophilic residue pattern on interleukin-10.

Authors:  Guoying Ni; Yuejian Wang; Scott Cummins; Shelley Walton; Kate Mounsey; Xiaosong Liu; Ming Q Wei; Tianfang Wang
Journal:  Hum Vaccin Immunother       Date:  2016-09-29       Impact factor: 3.452

6.  Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to the extracellular IL-22R1 chain.

Authors:  Brandi C Jones; Naomi J Logsdon; Mark R Walter
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-03-21

7.  Design and analysis of rhesus cytomegalovirus IL-10 mutants as a model for novel vaccines against human cytomegalovirus.

Authors:  Naomi J Logsdon; Meghan K Eberhardt; Christopher E Allen; Peter A Barry; Mark R Walter
Journal:  PLoS One       Date:  2011-11-21       Impact factor: 3.240

8.  A model of the ternary complex of interleukin-10 with its soluble receptors.

Authors:  Sergei Pletnev; Eugenia Magracheva; Alexander Wlodawer; Alexander Zdanov
Journal:  BMC Struct Biol       Date:  2005-06-28

9.  Investigation the Possibility of Using Peptides with a Helical Repeating Pattern of Hydro-Phobic and Hydrophilic Residues to Inhibit IL-10.

Authors:  Guoying Ni; Shu Chen; Yuedong Yang; Scott F Cummins; Jian Zhan; Zhixiu Li; Bin Zhu; Kate Mounsey; Shelley Walton; Ming Q Wei; Yuejian Wang; Yaoqi Zhou; Tianfang Wang; Xiaosong Liu
Journal:  PLoS One       Date:  2016-04-21       Impact factor: 3.240
  9 in total

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