Literature DB >> 11716118

Rapid diagnosis of leishmaniasis by fluorogenic polymerase chain reaction.

G Wortmann1, C Sweeney, H S Houng, N Aronson, J Stiteler, J Jackson, C Ockenhouse.   

Abstract

A fluorescent DNA probe (LEIS.P1) specific for a conserved region of the small-subunit ribosomal RNA gene of Leishmania and a pair of flanking primers (LEIS.U1 and LEIS.L1) were designed for use in a fluorogenic polymerase chain reaction. Optimal assay conditions with zero background were established to detect low levels of Leishmania from clinical samples. By use of this assay, we amplified DNA from 27 strains of cultured Leishmania (both Old and New World strains) and selectively amplified Leishmania DNA from 12 paraffin-embedded human biopsy samples and 3 fresh human skin biopsy specimens. For the fresh human tissue biopsies, the turnaround time from biopsy to test result was < 24 hr. No amplification was detected in negative control samples (including the kinetoplastid protozoa Trypanosoma rangelli and Crithidia fasiculata). This assay provides a specific and rapid diagnostic modality to detect infection with Leishmania.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 11716118     DOI： 10.4269/ajtmh.2001.65.583

Source DB:  PubMed          Journal:  Am J Trop Med Hyg        ISSN： 0002-9637            Impact factor:   2.345


  24 in total

Review 1.  Laboratory diagnosis of visceral leishmaniasis.

Authors:  Shyam Sundar; M Rai
Journal:  Clin Diagn Lab Immunol       Date:  2002-09

2.  Lipsosomal amphotericin B for treatment of cutaneous leishmaniasis.

Authors:  Glenn Wortmann; Michael Zapor; Roseanne Ressner; Susan Fraser; Josh Hartzell; Joseph Pierson; Amy Weintrob; Alan Magill
Journal:  Am J Trop Med Hyg       Date:  2010-11       Impact factor: 2.345

3.  Quantitative nucleic acid sequence-based assay as a new molecular tool for detection and quantification of Leishmania parasites in skin biopsy samples.

Authors:  Wendy F van der Meide; Gerard J Schoone; William R Faber; Jim E Zeegelaar; Henry J C de Vries; Yusuf Ozbel; Rudy F M Lai A Fat; Leíla I A R C Coelho; Masoom Kassi; Henk D F H Schallig
Journal:  J Clin Microbiol       Date:  2005-11       Impact factor: 5.948

4.  The Brief Case: Cutaneous Sporotrichosis in an Immunocompetent Patient after Travel to Peru.

Authors:  Dorit Wolff; Torsten Feldt; Julia Reifenberger; Heidi Sebald; Christian Bogdan
Journal:  J Clin Microbiol       Date:  2018-10-25       Impact factor: 5.948

5.  Detection, differentiation, and quantitation of pathogenic leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay.

Authors:  Alexandra Schulz; Katja Mellenthin; Gabriele Schönian; Bernhard Fleischer; Christian Drosten
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

6.  Carcinoma of the lip five years after bone marrow transplantation.

Authors:  M Ardore; M Berrone; G Marchitto; S Gandolfo; M Pentenero
Journal:  Ann Stomatol (Roma)       Date:  2015-12-01

7.  Comparison of parasitological, serological, and molecular tests for visceral leishmaniasis in HIV-infected patients: a cross-sectional delayed-type study.

Authors:  Gláucia Fernandes Cota; Marcos Roberto de Sousa; Betânia Mara de Freitas Nogueira; Luciana Inácia Gomes; Edward Oliveira; Tália Santana Machado Assis; Andréa Laender Pessoa de Mendonça; Bruna Fernandes Pinto; Juliana Wilke Saliba; Ana Rabello
Journal:  Am J Trop Med Hyg       Date:  2013-07-08       Impact factor: 2.345

8.  Detection and identification of Leishmania species from clinical specimens by using a real-time PCR assay and sequencing of the cytochrome B gene.

Authors:  Françoise Foulet; Françoise Botterel; Pierre Buffet; Gloria Morizot; Danièle Rivollet; Michèle Deniau; Francine Pratlong; Jean-Marc Costa; Stéphane Bretagne
Journal:  J Clin Microbiol       Date:  2007-05-02       Impact factor: 5.948

9.  Comparison between quantitative nucleic acid sequence-based amplification, real-time reverse transcriptase PCR, and real-time PCR for quantification of Leishmania parasites.

Authors:  Wendy van der Meide; Jorge Guerra; Gerard Schoone; Marit Farenhorst; Leíla Coelho; William Faber; Inge Peekel; Henk Schallig
Journal:  J Clin Microbiol       Date:  2007-10-24       Impact factor: 5.948

Review 10.  Gulf war servicemen and servicewomen: the long road home and the role of health care professionals to enhance the troops' health and healing.

Authors:  Robin B McFee
Journal:  Dis Mon       Date:  2008-05       Impact factor: 3.800
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.